Ethylene Glycol Metabolism in the Acetogen Acetobacterium woodii

Journal of Bacteriology
Dragan TrifunovićVolker Müller

Abstract

The acetogenic bacterium Acetobacterium woodii is able to grow by the oxidation of diols, such as 1,2-propanediol, 2,3-butanediol, or ethylene glycol. Recent analyses demonstrated fundamentally different ways for oxidation of 1,2-propanediol and 2,3-butanediol. Here, we analyzed the metabolism of ethylene glycol. Our data demonstrate that ethylene glycol is dehydrated to acetaldehyde, which is then disproportionated to ethanol and acetyl coenzyme A (acetyl-CoA). The latter is further converted to acetate, and this pathway is coupled to ATP formation by substrate-level phosphorylation. Apparently, the product ethanol is in part further oxidized and the reducing equivalents are recycled by reduction of CO2 to acetate in the Wood-Ljungdahl pathway. Biochemical data as well as the results of protein synthesis analysis are consistent with the hypothesis that the propane diol dehydratase (PduCDE) and CoA-dependent propionaldehyde dehydrogenase (PduP) proteins, encoded by the pdu gene cluster, also catalyze ethylene glycol dehydration to acetaldehyde and its CoA-dependent oxidation to acetyl-CoA. Moreover, genes encoding bacterial microcompartments as part of the pdu gene cluster are also expressed during growth on ethylene glycol, ar...Continue Reading

References

Jan 1, 1986·Journal of Bacteriology·D M Ivey, L G Ljungdahl
Feb 15, 1986·Archives of Biochemistry and Biophysics·M G Hartmanis, T C Stadtman
Dec 1, 1972·The American Journal of Clinical Nutrition·M P Bryant
Dec 1, 1972·Applied Microbiology·C F GonzalezM A Zeitoun
Dec 1, 1969·Japanese Journal of Microbiology·T MitsuokaS Yamamoto
Apr 11, 2003·Biochimica Et Biophysica Acta·Helmut Görisch
Feb 1, 1963·Journal of Bacteriology·L W GASTON, E R STADTMAN
Nov 7, 2003·Applied and Environmental Microbiology·Volker Müller
Jul 1, 1981·Applied and Environmental Microbiology·W H Caskey, W A Taber
Jul 1, 1989·Applied and Environmental Microbiology·H BuschhornG Gottschalk
Apr 2, 2008·Annals of the New York Academy of Sciences·Harold L DrakeSteven L Daniel
Sep 20, 2008·Biochimica Et Biophysica Acta·Stephen W Ragsdale, Elizabeth Pierce
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eva Biegel, Volker Müller
Jul 20, 2012·The Journal of Biological Chemistry·Kai Schuchmann, Volker Müller
Oct 2, 2012·Applied and Environmental Microbiology·Björn MückschelBernhard Hauer
Feb 18, 2014·Journal of Bacteriology·Elitza I TochevaGrant J Jensen
Oct 24, 2014·PLoS Computational Biology·Seth D AxenCheryl A Kerfeld
Nov 11, 2014·Nature Reviews. Microbiology·Kai Schuchmann, Volker Müller
Nov 12, 2014·Journal of Bacteriology·Kai SchuchmannVolker Müller
May 3, 2015·Applied and Environmental Microbiology·Verena HessVolker Müller

❮ Previous
Next ❯

Citations

May 18, 2018·Systematic Biology·Tobias AndermannAlexandre Antonelli
Aug 24, 2018·Environmental Microbiology·Mats LechtenfeldVolker Müller
Sep 23, 2018·Applied and Environmental Microbiology·Judith Dönig, Volker Müller
Jul 14, 2018·Environmental Microbiology·Florian KrempVolker Müller
Apr 14, 2019·Applied and Environmental Microbiology·Anne E BookerMichael J Wilkins
May 19, 2019·Genes·Manuel SalvadorJose I Jimenez
Nov 13, 2019·Environmental Microbiology Reports·Dragan TrifunovićVolker Müller
Jun 18, 2017·Biochemical Society Transactions·Abulikemu AbudukelimuHans V Westerhoff
May 7, 2020·Frontiers in Microbiology·Jiakang RuYu Yang
Oct 8, 2019·Environmental Science. Processes & Impacts·Brandon C McAdamsJ Alexandra Hakala
Nov 13, 2020·Frontiers in Microbiology·Nilanjan Pal ChowdhuryVolker Müller
Dec 3, 2020·Frontiers in Microbiology·Clodagh M CarrAlan D W Dobson
Dec 2, 2020·The Science of the Total Environment·Xiaowen ZhuAiduan Borrion
Apr 3, 2021·Applied Biochemistry and Biotechnology·Writtik MaityAmrita Roy
Apr 6, 2021·Environmental Microbiology·Dragan TrifunovićVolker Müller
Aug 28, 2021·Environmental Microbiology·Jimyung Moon, Volker Müller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.