PMID: 11607429Oct 15, 1993Paper

Ethylene induces de novo synthesis of chlorophyllase, a chlorophyll degrading enzyme, in Citrus fruit peel

Proceedings of the National Academy of Sciences of the United States of America
T TrebitshJ Riov

Abstract

Chlorophyllase (Chlase; EC 3.1.1.14) was extracted from plastid fractions of ethylene-treated orange fruit peel and purified 400-fold to homogeneity by gel filtration, hydrophobic chromatography, and preparative SDS/PAGE of nonheated protein. SDS/PAGE of nonheated purified enzyme indicated that Chlase activity is associated with a single protein band migrating at an apparent molecular mass of 25 kDa whereas the heated purified enzyme had a molecular mass of 35 kDa. The N-terminal sequence of the purified protein was determined. The purified enzyme was used as an immunogen for raising antibodies in rabbits. The antiserum was highly specific and on Western blots recognized both the heated and the nonheated form of Chlase. The antibodies also recognized the solubilized enzyme, as shown by an immunoprecipitation assay and by antigen-antibody capture assays in microtiter plates. Treatment with ethylene, which enhances degreening, increased Chlase activity 12-fold. Immunoblot analyses of crude extracts from ethylene-treated fruit detected a strong signal of the Chlase protein, while only a trace level of the enzyme protein could be detected in air. Gibberellin A3 and N6-benzyladenine partly counteracted the ethylene-induced increase ...Continue Reading

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Citations

May 26, 2005·Archives of Biochemistry and Biophysics·Kiani A J ArkusJoseph M Jez
Oct 25, 2000·Trends in Plant Science·K I TakamiyaH Ohta
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Philippe MatileHoward Thomas
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Apr 29, 2006·Analytical Biochemistry·Kiani A J Arkus, Joseph M Jez
May 8, 2015·Molecular Genetics and Genomics : MGG·Chunzhen ChengGuangyan Zhong
Dec 21, 2010·Biochimica Et Biophysica Acta·Stefan Hörtensteiner, Bernhard Kräutler
Oct 20, 2015·Journal of Agricultural and Food Chemistry·Yi-Li ChouJei-Fu Shaw
Feb 1, 2017·Journal of the Science of Food and Agriculture·Sunny George GwanpuaAnnemie H Geeraerd
Mar 26, 2002·Journal of Experimental Botany·Stefan Hörtensteiner, Urs Feller
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Jul 30, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chih-Chung YenJei-Fu Shaw
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Oct 2, 2003·Journal of Agricultural and Food Chemistry·Yaron SitritYosef Mizrahi

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