Ethylene signaling is critical for synergid cell functional specification and pollen tube attraction

The Plant Journal : for Cell and Molecular Biology
Cheng ZhangZhongnan Yang

Abstract

ETHYLENE INSENSITIVE 3 (EIN3) is a key regulator of ethylene signaling, and EIN3-BINDING F-BOX1 (EBF1) and EBF2 are responsible for EIN3 degradation. Previous reports have shown that the ebf1 ebf2 double homozygous mutant cannot be identified. In this study, the genetic analysis revealed that the ebf1 ebf2 female gametophyte is defective. The pollination experiment showed that ebf1 ebf2 ovules failed to attract pollen tubes. In female gametophyte/ovule, the synergid cell is responsible for pollen tube attraction. Observation of the pEIN3::EIN3-GFP transgenic lines showed that EIN3 signal was over-accumulated at the micropylar end of ebf1 ebf2 female gametophyte. The overexpression of stabilized EIN3 in synergid cell led to the defect of pollen tube guidance. These results suggested that the over-accumulated EIN3 in ebf1 ebf2 synergid cell blocks its pollen tube attraction which leads to the failure of ebf1 ebf2 homozygous plant. We identified that EIN3 directly activated the expression of a sugar transporter, SENESCENCE-ASSOCIATED GENE29 (SAG29/SWEET15). Overexpression of SAG29 in synergid cells blocked pollen tube attraction, suggesting that SAG29 might play a role in ethylene signaling to repel pollen tube entry. Taken togeth...Continue Reading

References

Aug 1, 1990·The Plant Cell·D R SmythE M Meyerowitz
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·K L ClarkC Chang
Jun 3, 1998·Applied and Environmental Microbiology·B B ChristensenS Molin
Jan 29, 2000·Nucleic Acids Research·S Kosugi, Y Ohashi
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A B Bleecker, H Kende
Nov 14, 2002·Annual Review of Genetics·Gary N Drews, Ramin Yadegari
Jul 29, 2003·Journal of Plant Research·Tetsuya Higashiyama
Apr 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jennifer M GagneRichard D Vierstra
Oct 7, 2004·Plant Physiology·Brad M BinderAnthony B Bleecker
Apr 7, 2005·Journal of Molecular Biology·Kazuhiko YamasakiShigeyuki Yokoyama
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Joshua G SteffenGary N Drews
Mar 3, 2010·Annual Review of Plant Biology·Wei-Cai YangYan-Hong Chen
Nov 26, 2010·Nature·Li-Qing ChenWolf B Frommer
Nov 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chuanli JuCaren Chang
May 9, 2014·Nature Communications·Yue LouZhong-Nan Yang
Apr 29, 2015·Cell·Daisuke MaruyamaTetsuya Higashiyama
Apr 14, 2016·Current Biology : CB·Akane G MizukamiTetsuya Higashiyama

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Citations

Mar 23, 2021·Horticulture Research·Huibin WangLingfei Xu
Feb 2, 2020·Plant Science : an International Journal of Experimental Plant Biology·Jing AnChristian Chervin
May 1, 2021·International Journal of Molecular Sciences·Ahmed G GadYing Miao
Jan 28, 2021·Journal of Integrative Plant Biology·Chaoqi WangXin Zhou

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