PMID: 7540046Jan 1, 1995Paper

Ethylenediaminetetraacetic acid in ammonium hydroxide for reducing decalcification time

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
C SandersonL Mayton

Abstract

Ethylenediaminetetraacetic acid (EDTA) solution is used to decalcify bone specimens for histological examination. Sodium hydroxide (NaOH) has been used to dissolve EDTA and to bring EDTA solutions to neutral pH. This solution, however, requires several weeks to decalcify bone specimens. We investigated a new decalcification fluid using concentrated ammonium hydroxide (NH4OH) to dissolve EDTA and to adjust the pH to neutral. Decalcification was performed using a magnetic stirrer with and without vacuum, or with a sonic cleaner. Decalcification end point was confirmed using both the weight loss and X-ray methods. After decalcification, specimens were processed through paraffin and sections were stained with hematoxylin and eosin. Decalcification employing NH4OH required an average of six days. Light microscopy indicated good retention of cellular detail.

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Citations

Sep 3, 2008·Calcified Tissue International·Rishard SalieMira Susa
Mar 1, 2012·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Shanna M WilsonMatthew B Wheeler
Jul 6, 2010·Acta histochemica·Juliana Dos Santos NevesPedro Duarte Novaes
Nov 7, 2007·The Spine Journal : Official Journal of the North American Spine Society·Mark L WangCholl W Kim
Oct 2, 2014·The Journal of Comparative Neurology·Maria MorellMichel André
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·M L StoverA C Lichtler
Nov 6, 2007·Brazilian Dental Journal·Dimitrius Leonardo PitolLaurelúcia Orive Lunardi
Apr 29, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J C AlersH van Dekken
May 26, 2018·Scientific Reports·Katie R WilsonMeenal Mehrotra
Jan 26, 1999·Clinical Orthopaedics and Related Research·E L Pesanti, J A Lorenzo
Nov 13, 2020·PloS One·Nadezhda V VorobievaAlexander S Graphodatsky
Oct 6, 2021·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Martin GestewitzTobias Albrecht

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