PMID: 6987492Jan 1, 1980Paper

Ethylnitrosourea-induced mutagenesis in asynchronous and synchronous Chinese hamster ovary cells

Mutation Research
R Goth-Goldstein, H J Burki

Abstract

The toxic and mutagenic activity of the alkylating carcinogen N-ethyl-N-nitrosourea (ENU) was studied in Chinese hamster ovary (CHO) cells. Cell killing and induction of 6-thioguanine-resistant (TGr) and ouabain-resistant (OUAr) mutants were determined as a function of ENU dose and treatment time in asynchronous cell populations. A dose-dependent induction of mutants was observed. The mutation frequency did not increase with longer than 30-min treatment times, implying that ENU breaks down rapidly in the cell. When synchronous populations of CHO cells obtained by mitotic detachment were treated with ENU at various times during the cell cycle, ENU-induced reproductive death was strongly dependent on the position in the cell cycle at the time of treatment, the time of highest sensitivity being the beginning of the S period. The pattern of mutation induction by ENU over the cell cycle was quite different from the pattern for cell killing. The induction of TGr mutants seemed to be independent of cell-cycle time. The induction of OUAr mutants was also independent of cell-cycle time after a low ENU dose; however, after a high ENU dose the frequency of OUAr mutants varied during the cell cycle, with a slight enhancement in B1 and a de...Continue Reading

References

Jul 1, 1976·Chemico-biological Interactions·A H Nias, B W Fox
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S P Hawkes, J C Bartholomew
Feb 9, 1978·Nature·A V CarranoJ L Minkler
Oct 1, 1977·Nature·J P O'Neill, A W Hsie
Jul 1, 1975·Cell·W Burke, W L Fangman
Jan 1, 1976·Mutation Research·P M Aebersold, H J Burki
Mar 1, 1976·Mutation Research·J H CarverL E Hopwood
Feb 1, 1980·Mutation Research·H J BurkiR D Wood
Nov 13, 2009·Bulletin of Experimental Biology and Medicine·D V GuryevO V Boeva

Citations

Feb 1, 1980·Mutation Research·H J BurkiR D Wood
Apr 1, 1987·Mutation Research·R Goth-Goldstein, M Hughes
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C TongG M Williams
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·R Montesano
Nov 1, 1995·Experimental Lung Research·K E DriscollJ M Carter
Jan 1, 1988·Environmental and Molecular Mutagenesis·G J SmithK S Bentley

Related Concepts

Cell Cycle
Cricetulus
Cytological Techniques
Drug Resistance
Ethylnitrosourea
Cricetus
Clastogens
Nitrosourea Compounds
Ouabain
Ovary

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