PMID: 11607585Oct 24, 1995Paper

Etiolated cells of Chlamydomonas reinhardtii: choice material for characterization of mitochondrial membrane polypeptides

Proceedings of the National Academy of Sciences of the United States of America
P BennounM Delosme

Abstract

We have investigated a light-conditional mutant of Chlamydomonas reinhardtii (J12) that is unable to synthesize chlorophyll in the dark with the aim of characterizing the mitochondrial membrane polypeptides of this alga. A crude membrane fraction derived from etiolated cells was analyzed by gel electrophoresis, immunoblot analysis, and pulse-labeling in the presence of specific protein synthesis inhibitors. This fraction contained both mitochondrial and etioplast membranes, and the latter contained appreciable amounts of subunits of the cytochrome b6f complex. The mitochondria-encoded subunit 1 of cytochrome-c oxidase called COX1 was identified, and its synthesis was detected in this membrane fraction. The redox-difference spectra of mitochondrial cytochromes were studied in whole cells and membrane fractions, in both respiratory-competent and -deficient strains. Mitochondrial membranes could be further purified after sucrose gradient centrifugation. The use of etiolated cells and their membrane extracts, in association with appropriate methodologies, opens ways to study the molecular genetics of mitochondria in C. reinhardtii and allows us to address the question of the cooperation established between the three genetic compart...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N H Chua, P Bennoun
Aug 1, 1990·Plant Molecular Biology·D P MaS E Hasnain
Apr 1, 1989·The Journal of Cell Biology·R F MatagneF Sluse
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J E BoyntonE H Harris
Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D S Gorman, R P Levine

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Citations

May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Elizabeth H Harris
Feb 15, 2005·Plant Physiology and Biochemistry : PPB·Einar J Stauber, Michael Hippler
Jul 19, 2000·The Journal of Biological Chemistry·X Pérez-MartínezD González-Halphen
Dec 10, 2000·The Journal of Biological Chemistry·X Pérez-MartínezD González-Halphen
Aug 29, 1997·The Journal of Biological Chemistry·Y PierreJ L Popot
Oct 24, 1998·Biochimica Et Biophysica Acta·M HipplerJ D Rochaix

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