Etiologic features of diarrheagenic microbes in stool specimens from patients with acute diarrhea in Thailand.

Scientific Reports
Kazuhisa OkadaShigeyuki Hamada

Abstract

Many microbial species have been recognized as enteropathogens for humans. Here, we predicted the causative agents of acute diarrhea using data from multiplex quantitative PCR (qPCR) assays targeting 19 enteropathogens. For this, a case-control study was conducted at eight hospitals in Thailand. Stool samples and clinical data were collected from 370 hospitalized patients with acute diarrhea and 370 non-diarrheal controls. Multiple enteropathogens were detected in 75.7% and 13.0% of diarrheal stool samples using multiplex qPCR and bacterial culture methods, respectively. Asymptomatic carriers of enteropathogens were found among 87.8% and 45.7% of individuals by qPCR and culture methods, respectively. These results suggested the complexity of identifying causative agents of diarrhea. An analysis using the quantification cut-off values for clinical relevance drastically reduced pathogen-positive stool samples in control subjects from 87.8% to 0.5%, whereas 48.9% of the diarrheal stool samples were positive for any of the 11 pathogens. Among others, rotavirus, norovirus GII, Shigella/EIEC, and Campylobacter were strongly associated with acute diarrhea (P-value < 0.001). Characteristic clinical symptoms, epidemic periods, and age-r...Continue Reading

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Citations

Dec 19, 2020·International Journal of Food Microbiology·Piyada WangroongsarbOrapan Sripichai
May 11, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nutthawadee JampanilPattara Khamrin
Jul 16, 2021·Current Opinion in Infectious Diseases·James A Platts-Mills, Elizabeth T Rogawski McQuade

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Methods Mentioned

BETA
nucleic acid amplification
PCR

Software Mentioned

R
R Foundation for Statistical Computing

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