EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens

Veterinary Research
Wenyu LiXiaokai Song

Abstract

Avian coccidian parasites exhibit a high degree of site specificity in different Eimeria species. Although the underlying mechanism is unclear, an increasing body of evidence suggests that site specificity is due to the interaction between microneme proteins (MICs) and their receptors on the surface of target host cells. In this study, the binding ability of E. tenella MICs (EtMICs) to different intestinal tissue was observed by immunofluorescence to identify the key surface molecule on the parasite responsible for the site specificity. Subsequently, we identified the corresponding host-cell receptors by yeast two-hybrid screening and glutathione-S-transferase pull-down experiments and the distribution of these receptors was observed by immunofluorescence in chicken intestinal tissues. Finally, we evaluated the efficacy of receptor antiserum against the infection of E. tenella in chickens. The results showed that EtMIC3 could only bind to the caecum while EtMIC1, EtMIC2, and EtAMA1 did not bind to any other intestinal tissues. Anti-serum to EtMIC3 was able to block the invasion of sporozoites with a blocking rate of 66.3%. The receptors for EtMIC3 were BCL2-associated athanogene 1 (BAG1) and Endonuclease polyU-specific-like (EN...Continue Reading

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Citations

Jun 3, 2021·Animals : an Open Access Journal From MDPI·Marco A Juárez-EstradaRogelio A Alonso-Morales
Sep 5, 2021·Microbiology Spectrum·Ningning ZhaoXiaomin Zhao
Oct 12, 2021·Frontiers in Genetics·Peipei ChengFeng Gu
Oct 28, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Martin A LauxmannVeronica I Dodero

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Methods Mentioned

BETA
two-hybrid
pull-down
PCR
ELISA
protein assay
reverse transcription PCR
X-ray

Software Mentioned

big
TMpred
DUALhunter
BLAST
MacMaster
Protean of Lasergene
PI Predictor

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