Ets-1 promoter-associated noncoding RNA regulates the NONO/ERG/Ets-1 axis to drive gastric cancer progression

Oncogene
Dan LiQ Tong

Abstract

Emerging studies have indicated the essential functions of long noncoding RNAs (lncRNAs) during cancer progression. However, whether lncRNAs contribute to the upregulation of v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets-1), an established oncogenic protein facilitating tumor invasion and metastasis, in gastric cancer remains elusive. Herein, we identified Ets-1 promoter-associated noncoding RNA (pancEts-1) as a novel lncRNA associated with the gastric cancer progression via mining of publicly available datasets and rapid amplification of cDNA ends. RNA pull-down, RNA immunoprecipitation, in vitro binding, and RNA electrophoretic mobility shift assays indicated the binding of pancEts-1 to non-POU domain containing octamer binding (NONO) protein. Mechanistically, pancEts-1 facilitated the physical interaction between NONO and Ets related gene (ERG), resulting in increased ERG transactivation and transcription of Ets-1 associated with gastric cancer progression. In addition, pancEts-1 facilitated the growth and aggressiveness of gastric cancer cells via interacting with NONO. In gastric cancer tissues, pancEts-1, NONO, and ERG were upregulated and significantly correlated with Ets-1 levels. High levels of pancEts-1, NO...Continue Reading

References

Apr 1, 1993·Journal of Virology·A M ChumakovH P Koeffler
May 10, 1996·The Journal of Biological Chemistry·M HallierF Moreau-Gachelin
Feb 7, 2002·Biochemical and Biophysical Research Communications·Shinji NaitoIchiro Sekine
Apr 30, 2002·Seminars in Radiation Oncology·Mary Beth TerryMarilie D Gammon
Oct 16, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J FujimotoT Tamaya
Nov 6, 2002·FEBS Letters·Yaron Shav-Tal, Dov Zipori
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Hitoshi IshiguroYoshinobu Kubota
Apr 25, 2007·Molecular and Cellular Biology·Xuesen DongStephen J Lye
Sep 6, 2007·The Tohoku Journal of Experimental Medicine·Eiichiro YamaguchiTakeshi Nagayasu
Jun 7, 2011·Carcinogenesis·Susanne SchiffnerAnja-Katrin Bosserhoff
Sep 29, 2011·Seminars in Cell & Developmental Biology·Stryder M MeadowsPaul A Krieg
Jul 31, 2012·Biochemical and Biophysical Research Communications·Tomohiro YamauchiKiyoshi Furuichi
Nov 5, 2013·Nucleic Acids Research·Audrey M MichelPavel V Baranov
Feb 4, 2014·Virchows Archiv : an International Journal of Pathology·Cecília DurãesFátima Carneiro
Jun 27, 2014·PloS One·Mohamed KabboutMichael C Ostrowski
Apr 29, 2015·Oncogene·P Adamo, M R Ladomery
Sep 24, 2015·Seminars in Cancer Biology·Jürgen Dittmer
May 4, 2016·The Journal of Clinical Investigation·Zirong ChenLizi Wu

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Citations

Mar 14, 2020·Journal of Cellular and Molecular Medicine·Peifu FengMao Ye
Nov 28, 2018·Wiley Interdisciplinary Reviews. RNA·Kiyoshi Masuda, Yuki Kuwano
Dec 26, 2018·Journal of Cellular Biochemistry·Zhihui LiuXiaojie Song
Jul 30, 2020·Cancers·Neri MercatelliMaria Paola Paronetto
Mar 19, 2020·Journal of Experimental & Clinical Cancer Research : CR·Lidia ChelliniMaria Paola Paronetto
May 6, 2020·OncoTargets and Therapy·Xue LengFangzhou Song
Jan 26, 2021·Clinical and Translational Gastroenterology·Michael RohrDeborah A Altomare
Dec 29, 2020·Journal of Cellular and Molecular Medicine·Peifu FengMao Ye
Jan 1, 2020·RNA Biology·Yan HuangXiangting Wang
Jul 11, 2021·Journal of Cellular and Molecular Medicine·Rong SongShang-Jie Wu
Oct 15, 2021·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Zeng-Peng SunWei-Min Yi

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Methods Mentioned

BETA
xenografts
transfection
pull-down
immunoprecipitation
electrophoretic mobility shift assay
RIP
co-immunoprecipitation
PCR
xenograft
Co-IP

Software Mentioned

Coding Potential Assessment Tool ( CPAT )
UCSC Genome Browser

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