Ets-2 and p53 mediate cAMP-induced MMP-2 expression, activity and trophoblast invasion.

Reproductive Biology and Endocrinology : RB&E
Elsebeth Staun-RamEliezer Shalev

Abstract

We have previously shown that Matrix metalloproteinase (MMP) -2 is a key-enzyme in early trophoblast invasion and that Protein Kinase A (PKA) increases MMP-2 expression and trophoblast invasion. The aim of this study was to examine MMP -2 regulation by PKA in invasive trophoblasts: JAR choriocarcinoma cell-line and 6-8 w first trimester trophoblasts. The effect of Forskolin (PKA) on MMP-2 expression was assessed by Northern Blot and RT-PCR. Possible transcription factors binding to consensus MMP-2 promoter sequences in response to Forskolin, were detected by EMSA binding assay and their expression assessed by western blot analysis. Antisense transfection of relevant transcription factors was performed and the inhibitory effect assessed on MMP-2 expression (RT-PCR), secretion (zymography) and trophoblast invasiveness (transwell migration assay). We found that Forskolin increased MMP-2 mRNA in JAR cells within 24 hours, and induced binding to p53, Ets, C/EBP and AP-2. Transcription factors Ets-2, phospho- p53, C/EBP epsilon, C/EBP lambda and AP-2 alpha bound to their respective binding sequences in response to Forskolin and the expressions of these transcription factors were all elevated in Forskolin- treated cells. Inhibition of...Continue Reading

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Citations

Feb 3, 2012·Carcinogenesis·Yu-Lun LiaoWen-Chang Lin
Nov 17, 2010·Molecular and Cellular Biology·Andrew M ParrottMichael B Mathews
Sep 26, 2012·Experimental Diabetes Research·Liping ZhuJerry Yee
Dec 9, 2014·Cell Cycle·Martin FischerKurt Engeland
May 25, 2013·Neurobiology of Disease·Chen-Huan LinWoei-Cherng Shyu
Sep 4, 2016·Human Reproduction Update·Teena Kjb GamageJoanna L James
Oct 29, 2017·Cellular and Molecular Life Sciences : CMLS·Martin GausterAndreas Prokesch
Nov 25, 2017·Journal of Experimental & Clinical Cancer Research : CR·Bhushan Thakur, Pritha Ray
Feb 27, 2018·Biology of Reproduction·Michael J SoaresKhursheed Iqbal

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Methods Mentioned

BETA
FCS
X-ray
electrophoresis
transfection
reverse transcription-PCR
Assay
zymography
ESR

Software Mentioned

- Capt
BioCapt
TINA
Image Pro
Image
Bio

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