Ets transcription factors bind and transactivate the core promoter of the von Willebrand factor gene

Oncogene
J L SchwachtgenD Kerbiriou-Nabias

Abstract

von Willebrand factor (vWF) gene expression is restricted to endothelial cells and megakaryocytes. Previous results demonstrated that basal transcription of the human vWF gene is mediated through a promoter located between base pairs -89 and +19 (cap site: +1) which is functional in endothelial and non endothelial cells. Two DNA repeats TTTCCTTT correlating with inverted consensus binding sites for the Ets family of transcription factors are present in the -56/-36 sequence. In order to analyse whether these DNA elements are involved in transcription, human umbilical vein endothelial cells (HUVEC), bovine calf pulmonary endothelial cell line (CPAE), HeLa and COS cells were transfected with constructs containing deletions of the -89/+19 fragment, linked to the chloramphenicol acetyl transferase (CAT) reporter gene. The -60/+19 region exhibits significant promoter activity in HUVEC and CPAE cells only. The -42/+19 fragment is not active. Mutations of the -60/+19 promoter fragment in the 5' (-56/-49) Ets binding site abolish transcription in endothelial cells whereas mutations in the 3' (-43/-36) site does not. The -60/-33 fragment forms three complexes with proteins from HUVEC nuclear extracts in electrophoretic mobility shift ass...Continue Reading

Citations

Jun 27, 2000·Journal of Cellular Physiology·T NakanoY Sato
Feb 1, 2003·Gene·Tsuneyuki Oikawa, Toshiyuki Yamada
Aug 10, 1999·Critical Reviews in Oncology/hematology·U M Vischer, P de Moerloose
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·P W HewettJ Clifford Murray
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·E LelièvreB Vandenbunder
Jun 17, 2009·Journal de la Société de biologie·Alexandra Le Bras, Fabrice Soncin
May 1, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Marjan NassiriNadia Jahroudi
Aug 25, 2007·The Journal of Clinical Investigation·Konstantin Levay, Vladlen Z Slepak
Dec 25, 2009·BMC Developmental Biology·Vesna Nikolova-KrstevskiPeter Oettgen
May 23, 2009·Journal of Inflammation·Yumei ZhanPeter Oettgen
Dec 5, 1998·Biochemical and Biophysical Research Communications·P W HewettJ C Murray
Mar 18, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Matthew C SalangaPaul A Krieg
Sep 24, 2004·Cancer Genetics and Cytogenetics·Patricia GargalloIrene Larripa
Feb 28, 2002·Biochemical and Biophysical Research Communications·L Van RompaeyG Grosveld
Jan 16, 2016·Current Opinion in Hematology·Yaozu Xiang, John Hwa
Sep 29, 2011·Seminars in Cell & Developmental Biology·Stryder M MeadowsPaul A Krieg
Feb 15, 2001·Oncogene·V I Sementchenko, D K Watson
Jun 6, 2018·Blood·Junmei Chen, Dominic W Chung
Apr 13, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Anahita MojiriNadia Jahroudi
Sep 26, 2003·The Journal of Biological Chemistry·Nenad PetrovicLinda H Shapiro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.