Eukaryotic initiation factor 4B and the poly(A)-binding protein bind eIF4G competitively

Translation
Shijun Cheng, Daniel R Gallie

Abstract

The eukaryotic translation initiation factor (eIF) 4G functions as a scaffold protein that assembles components of the translation initiation complex required to recruit the 40S ribosomal subunit to an mRNA. Although many eukaryotes express two highly similar eIF4G isoforms, those in plants are highly divergent in size and sequence from one another and are referred to as eIF4G and eIFiso4G. Although the domain organization of eIFiso4G differs substantially from eIF4G orthologs in other species, the domain organization of plant eIF4G is largely unknown despite the fact that it is more similar in size and sequence to eIF4G of other eukaryotes. In this study, we show that eIF4G differs from eIFiso4G in that it contains two distinct interaction domains for the poly(A) binding protein (PABP) and eIF4B but is similar to eIFiso4G in having two eIF4A interaction domains. PABP and eIF4B bind the same N-terminal region of eIF4G as they do to a region C-proximal to the HEAT-1 domain in the middle domain of eIF4G, resulting in competitive binding between eIF4B and PABP to each site. eIF4G also differs from eIFiso4G in that no competitive binding was observed between PABP and eIF4A or between eIF4B and eIF4A to its HEAT-1-containing region....Continue Reading

References

Dec 1, 1995·Genes & Development·S Z Tarun, A B Sachs
Oct 1, 1996·Plant Molecular Biology·K S Browning
Aug 14, 1998·Molecular Cell·S E WellsA B Sachs
Jun 22, 2001·The Journal of Biological Chemistry·G W RogersW C Merrick
Jan 9, 2003·Plant Molecular Biology·Daniel R Gallie
Apr 12, 2003·The EMBO Journal·Déborah PrévôtThéophile Ohlmann
Nov 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Thomas Preiss, Matthias W Hentze
Jun 11, 2004·Annual Review of Biochemistry·Lee D Kapp, Jon R Lorsch
Oct 1, 2005·Quarterly Reviews of Biophysics·Assen Marintchev, Gerhard Wagner
Jun 29, 2006·The Journal of Biological Chemistry·Shijun Cheng, Daniel R Gallie

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Citations

Sep 18, 2013·BMC Evolutionary Biology·Shijun ChengDaniel R Gallie
Jan 1, 2014·Translation·Katsura Asano
May 29, 2015·The Arabidopsis Book·Karen S Browning, Julia Bailey-Serres
Nov 26, 2019·Biochemistry·Rishi Kumar MishraTanweer Hussain
Aug 18, 2021·Biochemical and Biophysical Research Communications·Yukie IwaoAkihiro Tomida

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Datasets Mentioned

BETA
ABO15893
M95747

Methods Mentioned

BETA
pull-down
PCR
X-ray

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