Eukaryotic Translation Initiation Factor 4 Gamma 1 (eIF4G1) is upregulated during Prostate cancer progression and modulates cell growth and metastasis

Scientific Reports
Praveen Kumar JaiswalHari K Koul

Abstract

eIF4G1, a critical component of the eIF4F complex, is required for cap-dependent mRNA translation, a process necessary for tumor growth and survival. However, the role of eIF4G1 has not been evaluated in Prostate Cancer (PCa). We observed an increased eIF4G1 protein levels in PCa tissues as compared to normal tissues. Analysis of the TCGA data revealed that eIF4G1 gene expression positively correlated with higher tumor grade and stage. Furthermore, eIF4G1 was over-expressed and or amplified, in 16% patients with metastatic PCa (SU2C/PCF Dream Team dataset) and in 59% of castration-resistant prostate cancer (CRPC) patients (Trento/Cornell/Broad dataset). We showed for the first time that eIF4G1 expression was increased in PCa and that increased eIF4G1 expression associated with tumor progression and metastasis. We also observed high protein levels of eIF4G1 in PCa cell lines and prostate tissues from the TRAMP model of PCa as compared to normal prostate cell line and prostate tissues from the wild type mice. Knockdown of eIF4G1 in PCa cells resulted in decreased Cyclin D1 and p-Rb protein level, cell cycle delay, reduced cell viability and proliferation, impaired clonogenic activity, reduced cell migration and decreased mRNA loa...Continue Reading

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Citations

Feb 10, 2019·International Journal of Cancer. Journal International Du Cancer·Vibha SinghArrigo De Benedetti
Aug 17, 2019·Learning & Memory·Ilona R Kats, Eric Klann
Sep 10, 2019·Cancer Cell International·Praveen Kumar JaiswalHari K Koul
Feb 15, 2019·Frontiers in Genetics·Greco HernándezMiguel A Jiménez-Ríos
Jul 15, 2020·International Journal of Molecular Sciences·Irene Dell'AnnoStefano Landi
Feb 2, 2021·Journal of Cellular and Molecular Medicine·Ying LuZengguang Xu
Aug 15, 2021·Nature Communications·Stefano GrossoAnne E Willis

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Methods Mentioned

BETA
PCR
Transgenic
transfection
Density Gradient Fractionation

Software Mentioned

tpsDIG2w64
cBioPortal
tpsUtil64
ImageJ
NIH ImageJ
GraphPad Prism5
ModFit LT

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