EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes.

Leukemia
J J M Van DongenEuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Abstract

Most consensus leukemia & lymphoma antibody panels consist of lists of markers based on expert opinions, but they have not been validated. Here we present the validated EuroFlow 8-color antibody panels for immunophenotyping of hematological malignancies. The single-tube screening panels and multi-tube classification panels fit into the EuroFlow diagnostic algorithm with entries defined by clinical and laboratory parameters. The panels were constructed in 2-7 sequential design-evaluation-redesign rounds, using novel Infinicyt software tools for multivariate data analysis. Two groups of markers are combined in each 8-color tube: (i) backbone markers to identify distinct cell populations in a sample, and (ii) markers for characterization of specific cell populations. In multi-tube panels, the backbone markers were optimally placed at the same fluorochrome position in every tube, to provide identical multidimensional localization of the target cell population(s). The characterization markers were positioned according to the diagnostic utility of the combined markers. Each proposed antibody combination was tested against reference databases of normal and malignant cells from healthy subjects and WHO-based disease entities, respectiv...Continue Reading

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Citations

Jun 12, 2013·Trends in Biotechnology·Carlos E PedreiraUNKNOWN EuroFlow Consortium
Sep 6, 2012·Leukemia·T KalinaUNKNOWN EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Feb 6, 2013·Current Opinion in Hematology·Elaine Coustan-Smith, Dario Campana
Nov 20, 2012·Bioanalysis·Ruth M Barnard
Jul 13, 2013·Blood Reviews·Kathy HeelWendy N Erber
Mar 29, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P ValentH-P Horny
May 24, 2014·Leukemia·M DreylingO Hermine
Oct 4, 2014·Current Hematologic Malignancy Reports·Scott ElyAjai Chari
May 8, 2013·Blood·Paulina MirkowskaJean-Pierre Bourquin
May 10, 2015·Leukemia & Lymphoma·Maria Christina MaioliLeandro S Thiago
Mar 26, 2014·Leukemia & Lymphoma·Annamaria BrioliGareth J Morgan
Sep 4, 2013·Leukemia & Lymphoma·Hans Erik JohnsenUNKNOWN Myeloma Stem Cell Network (MSCNET)
Jul 6, 2014·Leukemia & Lymphoma·Suellen Valadares MouraMaria S Pombo-de-Oliveira
Jul 28, 2013·Leukemia & Lymphoma·Ben-Zion Katz, Yair Herishanu
Jul 3, 2015·Leukemia & Lymphoma·Morie A Gertz, Francis K Buadi
Jun 21, 2015·Expert Review of Clinical Immunology·Ger T RijkersFrans J van Overveld
Oct 28, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Tomas KalinaAlberto Orfao
Feb 24, 2016·Current Hematologic Malignancy Reports·Taiga NishihoriKenneth H Shain
Jun 24, 2015·Cytometry. Part B, Clinical Cytometry·Juan Flores-MonteroAlberto Orfao
Jun 26, 2015·Cytometry. Part B, Clinical Cytometry·Nicole J GormleyGerald E Marti
Jan 27, 2015·Cytometry. Part B, Clinical Cytometry·Maria ArrozBruno Paiva
Apr 25, 2015·Cytometry. Part B, Clinical Cytometry·Maryalice Stetler-StevensonAndy C Rawstron
Jul 2, 2015·Cytometry. Part B, Clinical Cytometry·Fanny PojeroUNKNOWN EuroFlow group
Jan 13, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Milena HasanUNKNOWN Milieu Intérieur Consortium
Oct 11, 2011·Leukemia Research·Theresia M WestersUNKNOWN Working Party on Flow Cytometry in MDS of Dutch Society of Cytometry (NVC)
Jul 21, 2015·Bone Marrow Transplantation·A M SherrodR F Cornell

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Methods Mentioned

BETA
biopsies
flow cytometry
PCA
MDS
dissection
biopsy
FACS
flow
PCR
of

Software Mentioned

APS1
AmCyan EuroFlow
Infinicyt
EuroFlow SOP
LST
EuroFlow LST
PCA
ALOT
EuroFlow

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