Europium (III) chelate microparticle-based lateral flow immunoassay strips for rapid and quantitative detection of antibody to hepatitis B core antigen

Scientific Reports
Rong-Liang LiangYingsong Wu

Abstract

Quantitative hepatitis B core antigen (anti-HBc) measurements could play an important role in evaluating therapeutic outcomes and optimizing the antiviral therapy of chronic hepatitis B infection. In this study, we have developed a simple and rapid fluorescence point-of-care test based on a lateral flow immunoassay (LFIA) method integrated with Eu (III) chelate microparticles to quantitatively determine anti-HBc concentrations in serum. This assay is based on a direct competitive immunoassay performed on lateral flow test strips with an assay time of 15 min. The Eu (III) chelate microparticle-based LFIA assay could quantitatively detect anti-HBc levels with a limit of detection of 0.31 IU mL(-1), and exhibited a wide linear range (0.63-640 IU mL(-1)). The intra- and inter-assay coefficients of variation for anti-HBc were both less than 10% and a satisfactory dilution test and accuracy were demonstrated. There were no statistically significant differences in sensitivity or specificity in serum samples between the Eu (III) chelate microparticle-based LFIA strips and the Abbott Architect kit. A simple, rapid and effective quantitative detection of anti-HBc was possible using the Eu (III) chelate microparticle-based LFIA strips. Th...Continue Reading

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Citations

Jun 5, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Chaoman AngYu Zhang
May 8, 2021·Frontiers in Molecular Biosciences·A CassedyR O'Kennedy
Jan 7, 2020·ACS Applied Materials & Interfaces·Qingyun LiuFuyou Li
Dec 5, 2018·Chemical Reviews·Christine F MarkwalterDavid W Wright

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
Fluorescence

Software Mentioned

Excel
SPSS13
SPSS
Origin Pro7

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