Europium-labeled activity-based probe through click chemistry: absolute serine protease quantification using (153)Eu isotope dilution ICP/MS

Angewandte Chemie
Xiaowen YanQiuquan Wang

Abstract

Click and analyze: the titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with (153)Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one (153)Eu(NO(3))(3) isotopic standard.

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Citations

Mar 28, 2013·Analytical and Bioanalytical Chemistry·Xiaowen YanQiuquan Wang
Jan 30, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Julianne Martell, Eranthie Weerapana
Feb 14, 2016·Plant & Cell Physiology·Kyoko Morimoto, Renier A L van der Hoorn
Feb 24, 2016·The Journal of Organic Chemistry·Praveen K ChinthakindiPer I Arvidsson
Apr 16, 2014·Medicinal Research Reviews·Laizhong ChenMinyong Li
Mar 1, 2017·Physical Chemistry Chemical Physics : PCCP·Hong Yan ZouCheng Zhi Huang
Dec 8, 2016·Bioorganic & Medicinal Chemistry Letters·Laura E Edgington-MitchellNigel Bunnett
Aug 13, 2014·Angewandte Chemie·Jiajia DongK Barry Sharpless
Sep 26, 2012·Chembiochem : a European Journal of Chemical Biology·D Alexander ShannonEranthie Weerapana
May 1, 2015·Chemical Science·Arjun Narayanan, Lyn H Jones
Jun 30, 2019·Analytical Chemistry·Rui LiuYi Lv
Oct 24, 2014·Bioconjugate Chemistry·Yiqing ZhouZhengying Pan
Oct 19, 2012·Analytical Chemistry·Zhubao ZhangQiuquan Wang
Apr 12, 2019·Analytical Chemistry·Zhian HuXinrong Zhang

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