EUS-guided in vivo imaging of the porcine esophageal enteric nervous system by using needle-based confocal laser endomicroscopy

Gastrointestinal Endoscopy
Jason B SamarasenaKenneth J Chang

Abstract

The GI tract is innervated by the autonomic enteric nervous system, mainly composed of submucosal Meissner's plexus and myenteric Auerbach's plexus, which is essential for motility, blood flow regulation, and secretory functions. In vivo visualization of the esophageal enteric nervous system (EENS) during endoscopy has not been possible without invasive mucosal resection. This study aimed to visualize the EENS without mucosal resection, in vivo by using the novel probe, needle-based confocal laser-induced endomicroscopy (nCLE) with a fluorescence neuronal probe, NeuroTrace, under EUS guidance and to evaluate the feasibility of ex vivo imaging of the neuronal network in submucosal biopsy samples acquired at endoscopy. Four Yorkshire pigs were anesthetized and examined. In vivo experiment: During endoscopy, NeuroTrace was injected into the submucosa and muscularis propria of the middle and distal esophagus under EUS guidance, and nCLE imaging was performed. Ex vivo experiment: Submucosal tissue biopsy specimens from the porcine esophagus were obtained for ex vivo evaluation by using a "through-the-needle" forceps technique. After incubation of the samples in NeuroTrace solution, pCLE was used to visualize the EENS elements in the...Continue Reading

References

Oct 27, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R De Giorgio, M Camilleri
May 9, 2006·Gastroenterology·David GrundyJackie D Wood
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Dec 24, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C H KnowlesR De Giorgio
Nov 1, 2011·Digestive Diseases and Sciences·Ashish NimgaonkarGeorge Triadafilopoulos
Mar 7, 2012·Nature Reviews. Gastroenterology & Hepatology·John B Furness
Jun 4, 2014·Gastrointestinal Endoscopy·Jason B SamarasenaKenneth J Chang

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Citations

Oct 13, 2020·United European Gastroenterology Journal

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