Euxanthone suppresses tumor growth and metastasis in colorectal cancer via targeting CIP2A/PP2A pathway

Life Sciences
Ning WangJingjing Cao

Abstract

Colorectal cancer (CRC) accounts for over 600,000 deaths annually worldwide. Euxanthone is a flavonoid compound extracted from Polygala caudata, with documented anti-neoplastic actions. The current study aimed to determine the therapeutic potential of euxanthone in CRC. Cell Counting Kit-8 (CCK-8) assay was used to analyze the effect of euxanthone on the cell viability, and apoptosis was detected by the TUNEL assay. The in vitro migratory capacity was determined by wound healing and the invasiveness was assessed by Transwell assay. Western blotting was used to determine the level of relevant proteins. Furthermore, a CRC xenograft murine model was used to analyze the therapeutic efficacy of euxanthone in vivo. Isobaric tags for relative and absolute quantification (iTRAQ) was then performed to identify the potential targets of euxanthone. To validate the role of cancerous inhibitor of PP2A (CIP2A) in the anti-cancer effects of euxanthone, plasmid overexpressing CIP2A and shRNA targeting CIP2A were used in in vitro assays. Euxanthone decreased cell viability and increased apoptosis in CRC cells, in addition to restraining migration, invasion and EMT. Similarly, euxanthone also effectively suppressed tumor growth and pulmonary met...Continue Reading

Citations

Nov 15, 2018·International Journal of Molecular Sciences·Salvatore ChirumboloTaras Upyr
Aug 23, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Fei ShenJing Xu
Jan 11, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Wen LiDaqing Song
Nov 26, 2019·Journal of Ethnopharmacology·Marie-Aleth Lacaille-DuboisAnne-Claire Mitaine-Offer
Aug 13, 2021·Frontiers in Oncology·Dengqiang WuSufang Zhou

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