PMID: 15348675Sep 7, 2004Paper

Evaluating 3D bone tissue engineered constructs with different seeding densities using the alamarBlue assay and the effect on in vivo bone formation

Journal of Materials Science. Materials in Medicine
C E WilsonJ D De Bruijn

Abstract

Bone tissue engineering using patient derived cells seeded onto porous scaffolds has gained much attention in recent years. Evaluating the viability of these 3D constructs is an essential step in optimizing the process. The alamarBlue (aB) assay was evaluated for its potential to follow in vitro cell proliferation on architecturally standardized hydroxyapatite scaffolds. The impact of the aB assayed and seeding density on subsequent in vivo bone formation was investigated. Twelve scaffolds were seeded with various densities from 250 to 2.5x10(6) cells/scaffold and assay by aB at 5 time points during the 7-day culture period. Twelve additional scaffolds were seeded with 2.5x10(5) cells/scaffold. Two control and 2 aB treated scaffolds were subcutaneously implanted into each of 6 nude mice for 6 weeks. Four observers ranked bone formation using a pair wise comparison of histological sections form each mouse. The aB assay successfully followed cell proliferation, however, the diffusion kinetics of the 3D constructs must be considered. The influence of in vitro aB treatment on subsequent in vivo bone formation cannot be ruled out but was not shown to be significant in the current study. The aB assay appears to be quite promising for...Continue Reading

Citations

Jan 5, 2006·Journal of Biomedical Materials Research. Part a·V V MeretojaT O Närhi
Jul 16, 2008·Tissue Engineering. Part a·Warren L GraysonGordana Vunjak-Novakovic
Feb 4, 2009·Journal of Biomedical Materials Research. Part a·Steven M van GaalenWouter J A Dhert
Sep 8, 2010·Journal of Biomaterials Applications·Fabian LangenbachJörg Handschel
Apr 22, 2006·Journal of Biomedical Materials Research. Part a·Y F ZhouT M Lim
Jul 10, 2013·Journal of Periodontal Research·K NakamuraY Hara
Jan 8, 2015·Annals of Biomedical Engineering·Shigeo M Tanaka, Kohei Tachibana
Nov 23, 2011·ACS Applied Materials & Interfaces·Sreeja NarayananDeepthy Menon

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