Evaluating Different Virulence Traits of Klebsiella pneumoniae Using Dictyostelium discoideum and Zebrafish Larvae as Host Models

Frontiers in Cellular and Infection Microbiology
Andrés E MarcoletaRosalba Lagos

Abstract

Multiresistant and invasive hypervirulent Klebsiella pneumoniae strains have become one of the most urgent bacterial pathogen threats. Recent analyses revealed a high genomic plasticity of this species, harboring a variety of mobile genetic elements associated with virulent strains, encoding proteins of unknown function whose possible role in pathogenesis have not been addressed. K. pneumoniae virulence has been studied mainly in animal models such as mice and pigs, however, practical, financial, ethical and methodological issues limit the use of mammal hosts. Consequently, the development of simple and cost-effective experimental approaches with alternative host models is needed. In this work we described the use of both, the social amoeba and professional phagocyte Dictyostelium discoideum and the fish Danio rerio (zebrafish) as surrogate host models to study K. pneumoniae virulence. We compared three K. pneumoniae clinical isolates evaluating their resistance to phagocytosis, intracellular survival, lethality, intestinal colonization, and innate immune cells recruitment. Optical transparency of both host models permitted studying the infective process in vivo, following the Klebsiella-host interactions through live-cell imag...Continue Reading

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Citations

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Datasets Mentioned

BETA
BAA-1705
APGM01000001.1
CP014696.2

Methods Mentioned

BETA
confocal microscopy
transgenic
Fluorescence
phosphotransferase

Software Mentioned

GraphPad Prism
Kaptive
BLAST
Fiji
Artemis
PathogenFinder
ZEN
Kleborate
ImageJ
ProgressiveMauve BLAST

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