Nov 8, 2018

Evaluating DNA methylation age on the Illumina’s methylationEPIC BeadChip

BioRxiv : the Preprint Server for Biology
Radhika DhingraCavin Ward-Caviness

Abstract

DNA methylation age (DNAm age) has become a widely utilized epigenetic biomarker for the aging process. The Horvath method for determining DNAm age is perhaps the most widely utilized and validated DNA methylation age assessment measure. Horvath DNAm age is calculated based on methylation measurements at 353 loci which were present on Illumina’s 450k and 27k DNA methylation microarrays. With the increasing use of the more recently developed Illumina MethylationEPIC (850k) microarray, it is worth revisiting this widely used aging measure to evaluate differences in DNA methylation age estimation based on array design. Of the requisite 353 loci, 17 are missing from the current 850k microarray. Using 17 datasets with 27k, 450k, and/or 850k methylation data, we calculated and compared each sample’s epigenetic age estimated from all 353 loci required from the Horvath DNAm age calculator (full), and using only the 336 loci present on the 27k, 450k, and 850k arrays (reduced). In 450k/27k data, missing loci caused underestimation of epigenetic age when compared with the full clock. Underestimation of full epigenetic age grew from ages 0 to ~20, remaining stable thereafter (mean= -3.46 y, SD=1.13) years for individuals ≥20 years. Underes...Continue Reading

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Mentioned in this Paper

Biological Markers
DNA Methylation [PE]
Protein Methylation
Research Personnel
DNA Methylation
Evaluation
Study of Epigenetics
Methylation
Aging-Related Process
Aging

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