Evaluating methods for extraction of α-cellulose from leaves of Melaleuca quinquenervia for stable carbon and oxygen isotope analysis

Rapid Communications in Mass Spectrometry : RCM
Jacinta S GreerXin Song

Abstract

Purification of α-cellulose from plant tissues is commonly conducted to facilitate the reliable measurement of stable isotope ratios. Prior research has shown that different plant species and tissues react differently to standardised cellulose extraction techniques. Thus, no single method can be applied to all materials and careful consideration must be undertaken when selecting an extraction technique. In order to evaluate their suitability for use on Melaleuca quinquenervia leaves, a suite of eight different cellulose extraction techniques were tested. Leaves of this species are preserved in perched lakes on southeast Queensland's sand islands and are a focus of ongoing palaeoclimate research. Elemental analyser/isotope ratio mass spectrometry was used to measure stable carbon and oxygen isotopic ratios and sample composition was measured using Fourier transform infrared spectroscopy. We demonstrate that the standard Brendel extraction technique, particularly with a higher reagent volume and longer boiling time, produces cellulose with the lowest spread in isotopic ratios among replicates, and with the fewest impurities detected by Fourier transform infrared spectroscopy. We also show that pre-treating the leaves to extract l...Continue Reading

References

Nov 16, 2005·Analytical Chemistry·Julia B GaudinskiRoderick E Wasylishen
Feb 26, 2008·Analytical Chemistry·Kevin J AnchukaitisDaniel P Schrag
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Aaron F DiefendorfKatherine H Freeman
Dec 25, 2010·The New Phytologist·I Colin PrenticeGuohong Wang
Aug 24, 2012·The New Phytologist·Cristina Moreno-GutiérrezJosé Ignacio Querejeta
Mar 1, 2007·Functional Plant Biology : FPB·Margaret M Barbour

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.