Evaluating mice lacking serum carboxylesterase as a behavioral model for nerve agent intoxication

Toxicology Mechanisms and Methods
Emily N DunnC Linn Cadieux

Abstract

Mice and other rodents are typically utilized for chemical warfare nerve agent research. Rodents have large amounts of carboxylesterase in their blood, while humans do not. Carboxylesterase nonspecifically binds to and detoxifies nerve agent. The presence of this natural bioscavenger makes mice and other rodents poor models for studies identifying therapeutics to treat humans exposed to nerve agents. To obviate this problem, a serum carboxylesterase knockout (Es1 KO) mouse was created. In this study, Es1 KO and wild type (WT) mice were assessed for differences in gene expression, nerve agent (soman; GD) median lethal dose (MLD) values, and behavior prior to and following nerve agent exposure. No expression differences were detected between Es1 KO and WT mice in more than 34 000 mouse genes tested. There was a significant difference between Es1 KO and WT mice in MLD values, as the MLD for GD-exposed WT mice was significantly higher than the MLD for GD-exposed Es1 KO mice. Behavioral assessments of Es1 KO and WT mice included an open field test, a zero maze, a Barnes maze, and a sucrose preference test (SPT). While sex differences were observed in various measures of these tests, overall, Es1 KO mice behaved similarly to WT mice....Continue Reading

References

Jul 1, 1987·Pharmacology, Biochemistry, and Behavior·K RaffaeleJ McDonough
Jan 1, 1993·Neuroscience and Biobehavioral Reviews·J H McDonough, T M Shih
Jun 1, 1997·Southern Medical Journal·S OhbuS Hinohara
Dec 2, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·S Karanth, C Pope
Feb 26, 2003·European Journal of Pharmacology·Laetitia Prut, Catherine Belzung
Mar 24, 2004·Journal of Intensive Care Medicine·Sage W Wiener, Robert S Hoffman
Mar 3, 2005·Pharmacology, Biochemistry, and Behavior·Rose-Marie KarlssonJacqueline N Crawley
Feb 1, 2006·Basic & Clinical Pharmacology & Toxicology·Trond MyhrerPål Aas
Dec 22, 2006·Neurotoxicology·Pierre FilliatJean-Marc Collombet
Apr 23, 2008·Current Protocols in Neuroscience·Stephen C Heinrichs, George F Koob
Sep 15, 2010·Life Sciences·Sunita SharmaHolly Brown-Borg
Jul 30, 2011·Prehospital Emergency Care : Official Journal of the National Association of EMS Physicians and the National Association of State EMS Directors·UNKNOWN National Association of EMS Physicians
Aug 31, 2011·Chemical Research in Toxicology·Ellen G DuysenOksana Lockridge
Jan 11, 2012·Methods in Molecular Biology·David H Overstreet
Jun 26, 2013·Biochemical and Biophysical Research Communications·Kosuke SaitoE James Squires
Aug 2, 2013·Biology of Sex Differences·Joshua C KwekelJames C Fuscoe
Aug 31, 2014·Neurotoxicology·Laxmikant S DeshpandeRobert J DeLorenzo
Oct 24, 2014·Frontiers in Behavioral Neuroscience·Tapan P PatelDavid F Meaney

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