Evaluating mixtures of 14 hygroscopic additives to improve antibody microarray performance

Analytical and Bioanalytical Chemistry
Sébastien BergeronDavid Juncker

Abstract

Microarrays allow the miniaturization and multiplexing of biological assays while only requiring minute amounts of samples. As a consequence of the small volumes used for spotting and the assays, evaporation often deteriorates the quality, reproducibility of spots, and the overall assay performance. Glycerol is commonly added to antibody microarray printing buffers to decrease evaporation; however, it often decreases the binding of antibodies to the surface, thereby negatively affecting assay sensitivity. Here, combinations of 14 hygroscopic chemicals were used as additives to printing buffers for contact-printed antibody microarrays on four different surface chemistries. The ability of the additives to suppress evaporation was quantified by measuring the residual buffer volume in open quill pins over time. The seven best additives were then printed either individually or as a 1:1 mixture of two additives, and the homogeneity, intensity, and reproducibility of both the spotted protein and of a fluorescently labeled analyte in an assay were quantified. Among the 28 combinations on the four slides, many were found to outperform glycerol, and the best additive mixtures were further evaluated by changing the ratio of the two additi...Continue Reading

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Citations

Sep 8, 2016·Microarrays·Anna S GerdtssonChrister Wingren
Oct 11, 2016·Lab on a Chip·A O OlanrewajuD Juncker
May 12, 2021·ACS Sensors·Frédéric NormandeauDavid Juncker
Sep 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Ricardo E AlvaradoLoïc Leroy

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