Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum

Nanoscale Research Letters
Arpita BasuSurekha Kundu

Abstract

Biosynthesis of gold nanoparticles of distinct geometric shapes with highly functional protein coats without additional capping steps is rarely reported. This study describes green synthesis of protein-coated gold nanoparticles for the first time from the edible, mycorrhizal fungus Tricholoma crassum (Berk.) Sacc. The nanoparticles were of the size range 5-25 nm and of different shapes. Spectroscopic analysis showed red shift of the absorption maxima with longer reaction period during production and blue shift with increase in pH. These were characterized with spectroscopy, SEM, TEM, AFM, XRD, and DLS. The particle size could be altered by changing synthesis parameters. These had potent antimicrobial activity against bacteria, fungi, and multi-drug-resistant pathogenic bacteria. These also had inhibitory effect on the growth kinetics of bacteria and germination of fungal spores. These showed apoptotic properties on eukaryotic cells when tested with comet assays. Moreover, the particles are capped with a natural 40 kDa protein which was utilized as attachment sites for genes to be delivered into sarcoma cancer cells. The present work also attempted at optimizing safe dosage of these nanoparticles using hemolysis assays, for appl...Continue Reading

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Citations

Oct 1, 2018·Nanoscale Research Letters·Mei-Xia ZhaoZhi-Qiang Zhang
Dec 15, 2019·Antibiotics·Abdulkader MasriRuqaiyyah Siddiqui
Oct 26, 2018·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jafar JalaeiMehdi Fazeli
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea RónaváriMonika Kiricsi
May 6, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Xing Yue XuYeon-Ju Kim

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Methods Mentioned

BETA
transmission
atomic
X-ray
scanning
AFM
surface plasmon resonance
transverse plasmon resonance
transmission electron microscopy
Bragg diffraction
Assay

Software Mentioned

OLYMPUS
MEASURE IT

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