Evaluating the cytometric detection and enumeration of the wine bacterium, Oenococcus oeni

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Louise BartleJames S Paterson

Abstract

Flow cytometry is a high throughput tool for determining microbial abundance in a range of medical, environmental and food-related samples. For wine, determining the abundance of Saccharomyces cerevisiae is well-defined and reliable. However, for the most common wine bacterium, Oenococcus oeni, using flow cytometry to determine cell concentration poses some challenges. O. oeni most often occurs in doublets or chains of varying lengths that can be greater than 7 cells. This wine bacterium is also small, at 0.2-0.6 μm, and may exhibit a range of morphologies including binary fission and aggregated complexes. This work demonstrates a straightforward approach to determining the suitability of flow cytometry for the chain-forming bacteria, O. oeni, and considerations when using flow cytometry for the enumeration of small microorganisms (< 0.5 μm). This article is protected by copyright. All rights reserved.

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Related Concepts

Environment
Flow Cytometry
Food
Saccharomyces cerevisiae
Wine
Oenococcus oeni
Detection
Complex (molecular entity)
Cells
Bacteria

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