Evaluating the cytometric detection and enumeration of the wine bacterium, Oenococcus oeni

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Louise BartleJames S Paterson


Flow cytometry is a high throughput tool for determining microbial abundance in a range of medical, environmental and food-related samples. For wine, determining the abundance of Saccharomyces cerevisiae is well-defined and reliable. However, for the most common wine bacterium, Oenococcus oeni, using flow cytometry to determine cell concentration poses some challenges. O. oeni most often occurs in doublets or chains of varying lengths that can be greater than 7 cells. This wine bacterium is also small, at 0.2-0.6 μm, and may exhibit a range of morphologies including binary fission and aggregated complexes. This work demonstrates a straightforward approach to determining the suitability of flow cytometry for the chain-forming bacteria, O. oeni, and considerations when using flow cytometry for the enumeration of small microorganisms (< 0.5 μm). This article is protected by copyright. All rights reserved.


Oct 1, 1991·Oral Microbiology and Immunology·E A Thibodeau, C M Ford
Jan 1, 1997·Methods in Enzymology·S B Haase, D J Lew
Mar 4, 2000·Applied and Environmental Microbiology·J C Cho, S J Kim
Apr 20, 2001·Journal of Microbiological Methods·P MalacrinòF Dellaglio
Jun 24, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Nobuyasu YamaguchiMasao Nasu
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker
Apr 24, 2008·BMC Microbiology·Johanna RoostaluTanel Tenson
May 30, 2008·Nature·Cara M SantelliKatrina J Edwards
Jun 20, 2008·Current Opinion in Microbiology·Kamila CzechowskaJan Roelof van der Meer
Jul 10, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Meredith E K CalvertLucy F Pemberton
Mar 10, 2011·PloS One·Jon Halvor Jonsrud KnutsenErik Boye
Aug 19, 2011·The ISME Journal·Jack A GilbertDawn Field
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Caroline E Abrahamse, Eveline J Bartowsky
Oct 20, 2012·Journal of Industrial Microbiology & Biotechnology·Mohammad SalmaH Alexandre
Aug 30, 2013·Nature·Emmanuelle Le ChatelierOluf Pedersen
Mar 13, 2014·PloS One·Emily R DavenportYoav Gilad
Jan 7, 2016·International Journal of Food Microbiology·Maria del Carmen PortilloAlbert Bordons
Jan 23, 2016·International Journal of Food Microbiology·Somnath ChakravortyRatan Gachhui
Jul 6, 2016·Frontiers in Microbiology·Aline FrossardMark O Gessner
Jan 28, 2017·Scientific Reports·Michiru NishitaYasuhiro Minami
May 30, 2017·Frontiers in Microbiology·Horatio H MorganMathabatha E Setati
Jan 1, 2018·Analytica Chimica Acta·Jason P BeechJonas O Tegenfeldt
Aug 15, 2018·FEMS Microbiology Ecology·Renee J SmithJames G Mitchell
Apr 5, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ger van den EnghDavid M Karl
Nov 20, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Antonio Cosma
Apr 29, 2020·Journal of Oral Microbiology·Jessica A P Carlson-JonesJames G Mitchell

Related Concepts

Flow Cytometry
Saccharomyces cerevisiae
Oenococcus oeni
Complex (molecular entity)

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

FEMS Microbiology Reviews
Eveline J Bartowsky
International Journal of Food Microbiology
Emilia Garcia-Moruno, Rosario Muñoz
© 2021 Meta ULC. All rights reserved