Evaluating the effects of allelochemical ferulic acid on Microcystis aeruginosa by pulse-amplitude-modulated (PAM) fluorometry and flow cytometry

Chemosphere
Rui WangDa-Qiang Yin

Abstract

We investigated the effects of allelochemical ferulic acid (FA) on a series of physiological and biochemical processes of blue-green algae Microcystis aeruginosa, in order to find sensitive diagnostic variables for allelopathic effects. Algal cell density was significantly suppressed by FA (0.31-5.17 mM) only after 48 h exposure. Inhibitions of photosynthetic parameters (F(v)/F(m) and F(v)'/F(m)') occurred more rapidly than cell growth, and the stimulation of non-photochemical quenching was observed as a feed-back mechanisms induced by photosystem II blockage, determining by PAM fluorometry. Inhibitions on esterase activity, membrane potential and integrity, as well as disturbance on cell size, were all detected by flow cytometry with specific fluorescent markers, although exhibiting varied sensitivities. Membrane potential and esterase activity were identified as the most sensitive parameters (with relatively lower EC50 values), and responded more rapidly (significantly inhibited only after 8 h exposure) than photosynthetic parameters and cell growth, thus may be the primary responses of cyanobacteria to FA exposure. The use of PAM fluorometry and flow cytometry for rapid assessment of those sensitive variables may contribute ...Continue Reading

References

Jun 1, 1977·Cryobiology·M D Persidsky, G S Baillie
Oct 1, 1992·Free Radical Biology & Medicine·E Graf
Apr 12, 2001·Plant Physiology·P MüllerK K Niyogi
Aug 30, 2001·Archives of Environmental Contamination and Toxicology·N M FranklinR P Lim
Mar 22, 2002·Trends in Biotechnology·Jennifer L StauberMerrin S Adams
Jul 20, 2002·Aquatic Toxicology·Rudi H RegelJustin D Brookes
May 20, 2003·Journal of Applied Microbiology·M D JohnstonR J W Lambert
Aug 3, 2004·Plant Physiology·Sarah Joshua, Conrad W Mullineaux
Dec 22, 2005·Ecotoxicology and Environmental Safety·Yang YuXiaoli Shi
Sep 8, 2009·International Journal of Food Microbiology·F M CamposT A Hogg

❮ Previous
Next ❯

Citations

Feb 15, 2018·Bulletin of Environmental Contamination and Toxicology·Xiaoxiong WangJing Chen
Jun 4, 2019·Environmental Science and Pollution Research International·Linfeng ChenWenhuai Wang
Apr 5, 2020·Environmental Science and Pollution Research International·Wenjing XuJiangtao Wang
Jul 14, 2020·Journal of Hazardous Materials·Xiaoqin ZhuHongying Hu
Apr 17, 2021·Environmental Science and Pollution Research International·Lixiao NiXuqing Chen
Jun 21, 2021·The Science of the Total Environment·Xiaoxiong WangJianqiang Liu
Mar 6, 2019·The Science of the Total Environment·Wei ZhaoXingZhang Luo
Jul 19, 2021·Mechanisms of Ageing and Development·Edward J CalabreseVittorio Calabrese

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.