Evaluating the effects of preanalytical variables on the stability of the human plasma proteome

Analytical Biochemistry
Maria E HassisKatherine E Williams

Abstract

High quality clinical biospecimens are vital for biomarker discovery, verification, and validation. Variations in blood processing and handling can affect protein abundances and assay reliability. Using an untargeted LC-MS approach, we systematically measured the impact of preanalytical variables on the plasma proteome. Time prior to processing was the only variable that affected the plasma protein levels. LC-MS quantification showed that preprocessing times <6h had minimal effects on the immunodepleted plasma proteome, but by 4 days significant changes were apparent. Elevated levels of many proteins were observed, suggesting that in addition to proteolytic degradation during the preanalytical phase, changes in protein structure are also important considerations for protocols using antibody depletion. As to processing variables, a comparison of single- vs double-spun plasma showed minimal differences. After processing, the impact ⩽3 freeze-thaw cycles was negligible regardless of whether freshly collected samples were processed in short succession or the cycles occurred during 14-17 years of frozen storage (-80 °C). Thus, clinical workflows that necessitate modest delays in blood processing times or employ different centrifugat...Continue Reading

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Citations

Apr 12, 2016·Proteomics. Clinical Applications·Bassel SabbaghPeter Findeisen
Sep 7, 2016·Expert Review of Proteomics·Junli DengYong Li
Feb 25, 2018·Proteomics. Clinical Applications·Juhura G AlmaziMatthew D Dun
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Jul 31, 2018·Expert Review of Proteomics·Viviana GrecoAndrea Urbani
Jul 6, 2019·Journal of Biophotonics·Lila LovergneGanesh D Sockalingum
Oct 1, 2019·EMBO Molecular Medicine·Philipp E GeyerMatthias Mann
Apr 14, 2020·Biopreservation and Biobanking·Dominik LermenMarike Kolossa-Gehring
Jun 15, 2019·Expert Review of Proteomics·N Corbacho-AlonsoM G Barderas
Jan 21, 2021·Clinical Proteomics·Patrick HalveyIshan Capila
Dec 29, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Yuko YamaguchiRichard D Semba
Dec 11, 2020·The American Journal of Clinical Nutrition·Tina E BrinkleyUNKNOWN Health, Aging, and Body Composition Study
Jul 4, 2021·Cell and Tissue Banking·Ana Caroline NeuberMárcia M C Marques
Sep 23, 2021·The Journal of Nutrition·Yuko YamaguchiRichard D Semba

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