Evaluating the involvement of alternative sigma factors SigF and SigG in Clostridium perfringens sporulation and enterotoxin synthesis.

Infection and Immunity
Jihong Li, Bruce A McClane

Abstract

Clostridium perfringens type A food poisoning is the second most commonly identified bacterial food-borne illness. Sporulation contributes to this disease in two ways: (i) most food-poisoning strains form exceptionally resistant spores to facilitate their survival of food-associated stresses, and (ii) the enterotoxin (CPE) responsible for the symptoms of this food poisoning is synthesized only during sporulation. In Bacillus subtilis, four alternative sigma factors mediate sporulation. The same four sigma factors are encoded by C. perfringens genomes, and two (SigE and SigK) have previously been shown to be necessary for sporulation and CPE production by SM101, a transformable derivative of a C. perfringens food-poisoning strain (K. H. Harry, R. Zhou, L. Kroos, and S. B. Melville, J. Bacteriol. 2009, 191:2728-2742). However, the importance of SigF and SigG for C. perfringens sporulation or CPE production had not yet been assessed. In the current study, after confirming that sporulating wild-type SM101 cultures produce SigF (from a tricistronic operon) and SigG, we prepared isogenic sigF- or sigG-null mutants. Whereas SM101 formed heat-resistant, phase-refractile spores, spore formation was blocked in the sigF- and sigG-null mut...Continue Reading

References

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Jan 31, 2007·Infection and Immunity·Jihong LiBruce A McClane
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Citations

Apr 16, 2013·Journal of Bacteriology·Kaori OhtaniTohru Shimizu
Aug 21, 2013·PLoS Genetics·Kelly A FimlaidAimee Shen
Oct 8, 2013·PLoS Genetics·Fátima C PereiraAdriano O Henriques
Feb 25, 2014·Molecular Microbiology·Glen P CarterDena Lyras
Aug 4, 2011·Microbial Pathogenesis·Daniel Paredes-SabjaMahfuzur R Sarker
Oct 7, 2014·Research in Microbiology·John C FreedmanBruce A McClane
Dec 30, 2014·Research in Microbiology·Prabhat K TalukdarMahfuzur R Sarker
May 28, 2013·Toxicon : Official Journal of the International Society on Toxinology·Michel R Popoff, Philippe Bouvet
Jun 25, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Janneke G AllaartAndrea Gröne
May 25, 2016·BMC Genomics·Lee-Yean LowJackie K Cheung
May 22, 2016·Avian Pathology : Journal of the W.V.P.A·Audrey CharleboisMarie Archambault
Feb 23, 2017·Infection and Immunity·Nozomu ObanaNobuhiko Nomura
Jan 30, 2015·Microbiology and Molecular Biology Reviews : MMBR·Mohab A Al-HinaiEleftherios T Papoutsakis
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Jihong LiBruce A McClane
Jun 15, 2014·Applied and Environmental Microbiology·David G KirkMiia Lindström
Aug 8, 2018·Emerging Microbes & Infections·Raymond Kiu, Lindsay J Hall
Apr 13, 2021·Virulence·Iman Mehdizadeh GohariBruce A McClane
Dec 21, 2019·Microbiology Spectrum·Aimee ShenDaniel Paredes-Sabja
Jun 25, 2015·Microbiology Spectrum·Vicki AdamsJulian I Rood
Jun 24, 2016·Microbiology Spectrum·Jihong LiBruce A McClane
Jun 25, 2015·Microbiology Spectrum·Peter Dürre
Sep 22, 2018·Microbiology Spectrum·Archana ShresthaBruce A McClane

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