Evaluating the Vaccine Potential of a Tetravalent Fusion Protein (rBm HAXT) Vaccine Antigen Against Lymphatic Filariasis in a Mouse Model

Frontiers in Immunology
Nikhil ChauhanRamaswamy Kalyanasundaram

Abstract

Lymphatic filariasis (LF) is a tropical parasitic infection of human transmitted by mosquitoes. Chronic infection results in severe physical disability in the infected patients. Although several potential vaccine antigens were identified by several groups, there are no licensed prophylactic vaccine to date against this infection in the human. Previous attempts from our laboratory to develop a trivalent prophylactic vaccine against LF showed that >90% protection could be achieved in rodent models. However, this trivalent vaccine gave only 35% protection in non-human primates. The major focus of this study was to develop a tetravalent prophylactic vaccine (rBmHAXT) and test the vaccine potential in a mouse model. We evaluated three different adjuvant formulations; alum, glucopyranosyl lipid adjuvant in stable emulsion (GLA/SE) alum (AL019), and mannosylated chitosan (MCA) to determine the optimum adjuvant formulation for rBmHAXT. Results presented in this study show that rBmHAXT + AL019 gave the highest rate of protection (>88%) against challenge infection, compared to rBmHAXT + AL007 (79%), rBmHAXT + MCA (79%) and controls. Analysis of the immune correlates of protection showed that all three adjuvants elicited high titer of ant...Continue Reading

References

Aug 1, 1996·Infection and Immunity·K A DimockP J Lammie
Mar 13, 1998·Molecular and Biochemical Parasitology·L KlimowskiC A Tripp
May 5, 1998·Molecular and Biochemical Parasitology·I GhoshA L Scott
Jun 24, 2003·Infection and Immunity·Kannan KunchithapauthamAlan L Scott
Nov 17, 2007·Nature Reviews. Immunology·Robert M AnthonyWilliam C Gause
Apr 29, 2008·Molecular and Biochemical Parasitology·James P HewitsonRick M Maizels
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gregory P PriebeGerald B Pier
May 23, 2009·Parasitology Research·Lorena Chiumiento, Fabrizio Bruschi
Jun 11, 2009·PLoS Neglected Tropical Diseases·Anandharaman VeerapathranRamaswamy Kalyanasundaram
Jan 30, 2010·Seminars in Immunopathology·Yinyao LinShabaana A Khader
Jun 28, 2011·Journal of Proteomics·Sirichit WongkamchaiVisith Thongboonkerd
Jan 1, 2010·Procedia in Vaccinology·Abhilash SamykuttyRamaswamy Kalyanasundaram
Jul 16, 2011·Research and Reports in Tropical Medicine·Ramaswamy Kalyanasundaram, Padmavathi Balumuri
Nov 22, 2011·Antioxidants & Redox Signaling·Michael C GretesP Andrew Karplus
Apr 25, 2012·Applied Biochemistry and Biotechnology·Setty Balakrishnan AnandPerumal Kaliraj
Jun 2, 2012·Indian Journal of Community Medicine : Official Publication of Indian Association of Preventive & Social Medicine·Prakash Kurubarahalli Patel
Aug 23, 2012·Antioxidants & Redox Signaling·David L WilliamsGustavo Salinas
Oct 6, 2012·Vaccine·Gajalakshmi DakshinamoorthyRamaswamy Kalyanasundaram
May 15, 2013·Current Opinion in Immunology·Pawan KumarJay K Kolls
Jun 15, 2013·Tropical Parasitology·Zinia T NujumS Muraleedharan Nair
Oct 23, 2013·PloS One·Gajalakshmi DakshinamoorthyRamaswamy Kalyanasundaram
Jan 24, 2014·Parasites & Vectors·Molly Brady, UNKNOWN Global Alliance to Eliminate Lymphatic Filariasis
Nov 18, 2014·PloS One·Gajalakshmi DakshinamoorthyRamaswamy Kalyanasundaram

❮ Previous
Next ❯

Citations

Jun 28, 2019·Current Topics in Medicinal Chemistry·Puvvada Kalpana Murthy
Oct 12, 2021·Expert Review of Vaccines·Vivek P ChavdaVandana Patravale
Nov 2, 2021·Expert Review of Vaccines·Rick M Maizels

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY692227.1
JF795950.1
AF319997.1
JF795955.1
was

Methods Mentioned

BETA
MDA
ELISA
flow cytometry

Software Mentioned

SPSS

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.