Evaluating urinary estrogen and progesterone metabolites using dried filter paper samples and gas chromatography with tandem mass spectrometry (GC-MS/MS)

BMC Chemistry
Mark NewmanFrank Z Stanczyk

Abstract

Measuring concentrations of metabolites of estradiol and progesterone in urine, instead of measuring serum concentrations, is common in research and also is used in patient care. The primary aim of this study was to demonstrate that analysis of urine samples dried on filter paper by gas chromatography with tandem mass spectrometry (GC-MS/MS) provides results similar to serum analyzed by radioimmunoassay (RIA). Secondary aims were to show that collection of four samples during the day (4-spot method) can be substituted for a 24-h collection, and that analysis of urine from dried samples is equivalent to liquid urine samples. This prospective observational study compared results of urine and serum analyses. Urine samples from women throughout the menstrual cycle and single samples from postmenopausal women were evaluated. Urine was collected onto filter paper and dried. Dried urine was extracted, hydrolyzed, and derivatized prior to analysis by GC-MS/MS. Hormone concentrations were normalized to creatinine. Single samples were used to compare results of 24-h urine collection to the 4-spot method from a separate population of women and men. A subset of these samples were used to compare results from dried urine to liquid urine. Th...Continue Reading

References

Jun 1, 1984·The Journal of Clinical Investigation·M FilicoriW F Crowley
Jun 10, 1998·American Journal of Epidemiology·K WallerB L Lasley
Mar 24, 2000·Endocrine-related Cancer·N M Probst-HenschB E Henderson
Mar 2, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Cedric Shackleton
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May 29, 2015·The European Journal of Contraception & Reproductive Health Care : the Official Journal of the European Society of Contraception·Judith RoosChristian Gnoth

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Citations

May 19, 2020·Metabolomics : Official Journal of the Metabolomic Society·Mohammad Khodadadi, Morteza Pourfarzam
Oct 7, 2020·American Journal of Human Biology : the Official Journal of the Human Biology Council·Virginia J Vitzthum
Nov 12, 2020·American Journal of Human Biology : the Official Journal of the Human Biology Council·Theresa E Gildner
Jan 19, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Yusuke OkamotoHiroshi Akiyama
Feb 16, 2021·Archives of Endocrinology and Metabolism·Paul AtkinsDavid Thompson
Mar 27, 2020·Clinical Biochemistry·Steven E Conklin, Claire E Knezevic
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Ra LeeBong-Chul Chung

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Methods Mentioned

BETA
urine collected
urine collection
contraception
hormone replacement therapy
urine collections

Software Mentioned

STAT
SAS

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