Evaluating wastewater-induced plant genotoxicity using randomly amplified polymorphic DNA

Environmental Toxicology
K M SwailehA Ezzughayyar

Abstract

Wastewater often contains genotoxic substances that can resist different stages of the treatment process. In the present study, randomly amplified polymorphic DNA technology was applied to evaluate the genotoxic effects of wastewater (treated and raw) irrigation on oat plants (Avena sativa). RAPD profiles obtained showed that both treated and raw wastewater (RWW) were having genotoxic effects on oat plants. This was apparent by the appearance/disappearance of bands in the treatments compared with the control plants. From the 15 primers used, 186 bands were obtained with an average of 12.4 bands per primer. Irrigating plants with RWW caused 51 new bands to appear and 19 to disappear. Treated wastewater (TWW) caused only 16 new bands and the loss of 17 bands. This makes TWW less genotoxic than RWW. The Euclidean distances shown on the dendrogram, revealed the presence of two clusters according to dissimilarity values. One cluster contained the control plants and those irrigated with TWW, whereas the second contained the plants irrigated with RWW. Similarity indices calculated between the treatments and the control plants showed that the control and the plants irrigated with TWW had a similarity index of 0.87, the control and plan...Continue Reading

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Citations

Jun 15, 2010·Environmental Science and Pollution Research International·Peng ZhanYingqiao Shi
Jan 12, 2013·Environmental Science and Pollution Research International·Milena PetriccioneClaudia Ciniglia
Oct 1, 2009·Arhiv za higijenu rada i toksikologiju·Jaouad El AssloujAbderraouf Hilali
Jul 9, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Tanmoy SarkarM P Reddy
Feb 19, 2013·Bulletin of Environmental Contamination and Toxicology·K M SwailehR M Hussein
Jan 26, 2016·Journal of Biochemical and Molecular Toxicology·Oluwakemi A RotimiElizabeth A Balogun
Nov 28, 2014·Environmental Science and Pollution Research International·Basem Shomar, Anne Dare
Oct 30, 2014·Archives of Environmental Contamination and Toxicology·Oguzhan Doganlar, Zeynep Banu Doganlar
Apr 3, 2013·Toxicology and Industrial Health·M Serdal SakcaliHüseyin Tombuloglu
Apr 14, 2012·Toxicology and Industrial Health·Filiz Aygun ErturkGuleray Agar
Dec 24, 2009·Human & Experimental Toxicology·Xiaolin ZhangShupei Cheng
Jun 19, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Zeynep B Doganlar

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