PMID: 9186830May 1, 1997Paper

Evaluation and comparison of different hepatitis C virus genotyping and serotyping assays

Journal of Hepatology
J H LeeS Zeuzem

Abstract

Evidence that the geno/subtype of hepatitis C virus (HCV) is predictive of the response to interferon-alpha therapy suggests that typing methods are clinically useful. In the present study, HCV isolates obtained from 74 patients with chronic hepatitis C were used to evaluate three genotyping and two serotyping assays. The reverse hybridization assay and the DNA immunoassay are based on immobilized type-specific probes for the 5'-noncoding and the core region, respectively. A third genotyping assay utilized type-specific primers for amplification of the core region. Serotyping assays detect type-specific antibodies of the nonstructural-4 region (enzyme immunoassay) or of the core and nonstructural-4 region (recombinant immunoblot assay). Gold standard geno/subtyping of HCV isolates was performed by sequence and phylogenetic analysis of the nonstructural-5B region. All genotyping systems amplified the respective target region of the HCV genome with high sensitivity. The reverse hybridization assay and the DNA immunoassay correctly identified HCV-1, -2, and -3. The DNA immunoassay misinterpreted all HCV-4 isolates as HCV-4 and -5 coinfection. In the type-specific amplification assay, coinfections of subtypes HCV-1a and HCV-3a with...Continue Reading

References

Feb 28, 1992·Biochemical and Biophysical Research Communications·S MoriK Shimotohno
Aug 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K YoshiokaT Morishima
Sep 1, 1991·The Journal of General Virology·T NakaoT Date
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Jun 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T TanakaM Kohara
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·L StuyverG Maertens
Feb 1, 1995·Annals of Internal Medicine·J B NousbaumC Bréchot
Sep 1, 1994·Computer Applications in the Biosciences : CABIOS·Y Van de Peer, R De Wachter
Jun 1, 1994·Journal of Virological Methods·S ViazovM Roggendorf
Jul 1, 1994·The Journal of General Virology·M SakamotoH Okamoto
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller

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Citations

Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S ZeuzemW K Roth
Jan 31, 2006·Transactions of the Royal Society of Tropical Medicine and Hygiene·Chuan-Mo LeeWei-Jen Chen
Jan 6, 2000·Journal of Hepatology·J M Pawlotsky
Jul 25, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Alexander C HaushoferHarald H Kessler
Jan 6, 2001·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·G BresciA Capria
Nov 15, 2002·Journal of Viral Hepatitis·P HalfonD Ouzan
Sep 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S ZeuzemU Hopf
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Beat HelblingUNKNOWN Investigators of the Swiss Association for the Study of the Liver
Sep 22, 2006·Journal of Clinical Microbiology·Linda CookKeith R Jerome
Apr 11, 2008·Journal of Clinical Microbiology·Jannick VerbeeckMarianne Van Brussel
Apr 28, 2001·Journal of Clinical Microbiology·P HalfonD Ouzan
Nov 28, 2002·Journal of Clinical Microbiology·Sandra S Richter
Jul 5, 2013·PloS One·Brendan JackaTanya Applegate
Mar 4, 2000·AJR. American Journal of Roentgenology·C F DietrichS Zeuzem
Mar 30, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S ZeuzemH C Thomas
Oct 21, 2006·Journal of Viral Hepatitis·Beat HelblingUNKNOWN Swiss Association for the Study of the Liver (SASL)
Jan 25, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S CampiottoA P Bernardini
Aug 5, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·G LodiC Scully
May 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Eva HerrmannStefan Zeuzem
Jun 11, 1999·The American Journal of Gastroenterology·A E ReidT J Liang
Dec 18, 2012·International Journal of Molecular Medicine·Qingxian CaiZhiliang Gao
Oct 9, 1999·Clinical Microbiology Reviews·M Arens

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