Evaluation and optimization of multiple fluorophore analysis of a Pseudomonas aeruginosa biofilm.

Journal of Microbiological Methods
Fiona J BairdJane E Hill

Abstract

Conventional laser scanning microscopy for multiple fluorescent stains can be a useful tool if the problems of autofluorescence and cross-talk are eliminated. The technique of spectral imaging was employed to unmix five different fluorophores - ranging in emission from 435 to 665 nm - applied to a Pseudomonas aeruginosa biofilm with overlapping spectra and which was not possible using traditional channel mode operation. Using lambda scanning and linear unmixing, the five fluorophores could be distinguished with regions of differentiation apparent.

References

Feb 8, 1989·Journal of Immunological Methods·K D KrenikG J Gleich
Jul 15, 1983·Analytical Biochemistry·A F Wilderspin, N M Green
Apr 15, 1995·Microscopy Research and Technique·P G GermrothR P Thompson
Feb 1, 1995·Cytometry·R J FlorijnA K Raap
Dec 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A LonginP A Bryon
Feb 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M OnoA Segawa
Apr 23, 2004·Journal of Microscopy·R H Berg
Sep 24, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·S M Stocks
Jan 25, 2007·Applied Microbiology and Biotechnology·Ming-Yuan ChenKuan-Yeow Show
Apr 3, 2007·Letters in Applied Microbiology·P S StewartB Pitts
Aug 23, 2008·Journal of Microbiological Methods·Oxana NosykFritz H Frimmel
Jul 17, 2010·Pathology·Gelareh FarshidDavid F Moffat
May 21, 2011·FEMS Immunology and Medical Microbiology·Liang YangSøren Molin

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Citations

Oct 3, 2013·PloS One·Han-Shin Kim, Hee-Deung Park
Jan 24, 2016·Analytical Biochemistry·I Randrianjatovo-GbalouC-E Marcato-Romain
Dec 29, 2013·Microbial Ecology·Lizziane Kretli WinkelströterElaine Cristina Pereira De Martinis
Mar 17, 2016·Journal of Microbiological Methods·Sebastian Schlafer, Rikke L Meyer
Mar 27, 2018·Annual Review of Analytical Chemistry·Yi WangK Scott Phillips
Feb 23, 2020·Journal of Diabetes Research·Xin LiManuela Martins-Green
Sep 10, 2015·Microbiology Spectrum·Michael J FranklinBrian Bothner

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