Evaluation and preservation of vascular architectures in decellularized whole rat kidneys.

Cryobiology
Haikao FengWendell Q Sun

Abstract

Organ transplantation is the gold standard treatment for end-stage organ failure. Due to the severe shortage of transplantable organs, only a tiny fraction of patients may receive timely organ transplantation every year. Decellularization-recellularization technology using allogeneic and xenogeneic organs is currently conceived to be a promising solution to generate functionally transplantable organs in vitro. This approach, however, still faces tremendous technological challenges, one of them being the ability to evaluate and preserve the integrity of vascular architectures upon decellularization and cryostorage of the whole organ matrices so that the off-the-shelf organ grafts are available on demand for clinical applications. In the present study, we report a Micro-CT imaging method for evaluating the integrity of vasculature of the decellularized whole organ scaffolds with/without freezing/thawing. The method uses radiopaque Microfil perfusion and x-ray fluoroscopy to acquire high-resolution angiography of the organ matrix. The whole rat kidney is decellularized using a new multistep perfusion protocol with the combined use of Triton X-100 and DNase. The decellularized kidney matrix is then cryopreserved after the pretreatm...Continue Reading

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Citations

Sep 7, 2021·Frontiers in Bioengineering and Biotechnology·Ifeolu AkinnolaAngela Panoskaltsis-Mortari
Nov 5, 2021·ACS Biomaterials Science & Engineering·Xu GaoQiongyu Guo

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