Evaluation and utility of mitochondrial ribosomal genes for molecular systematics of parasitic nematodes.

Parasites & Vectors
Abigail Hui En ChanUrusa Thaenkham

Abstract

Molecular advances have accelerated our understanding of nematode systematics and taxonomy. However, comparative analyzes between various genetic markers have led to discrepancies in nematode phylogenies. This study aimed to evaluate the suitability of using mitochondrial 12S and 16S ribosomal RNA genes for nematode molecular systematics. To study the suitability of mitochondrial 12S and 16S ribosomal RNA genes as genetic markers for nematode molecular systematics, we compared them with the other commonly used genetic markers, nuclear internal transcribed spacer 1 and 2 regions, nuclear 18S and 28S ribosomal RNA genes, and mitochondrial cytochrome c oxidase subunit 1 gene. After that, phylum-wide primers for mitochondrial 12S and 16S ribosomal RNA genes were designed, and parasitic nematodes of humans and animals from 75 taxa with 21 representative species were inferred through phylogenetic analyzes. Phylogenetic analyzes were carried out using maximum likelihood and Bayesian inference algorithms. The phylogenetic relationships of nematodes based on the mitochondrial 12S rRNA gene supported the monophyly of nematodes in clades I, IV, and V, reinforcing the potential of this gene as a genetic marker for nematode systematics. In ...Continue Reading

References

Jan 9, 1998·International Journal for Parasitology·M S BlouinJ B Dame
Mar 24, 1998·Nature·M L BlaxterW K Thomas
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Feb 24, 2007·Trends in Genetics : TIG·Mehrdad HajibabaeiDonal A Hickey
Apr 25, 2007·Nucleic Acids Research·Warren A Kibbe
Sep 17, 2008·Current Protocols in Bioinformatics·Julie D ThompsonDes G Higgins
May 2, 2009·Genetic Testing and Molecular Biomarkers·Gianmarco FerriGiovanni Beduschi
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Feb 14, 2014·Scientific Reports·Li YangRonghua Li
Apr 23, 2014·Molecular Ecology Resources·Laurence J ClarkeAlan Cooper
Mar 12, 2015·Parasitology Research·Jennifer K KetzisGraham Burgess
Nov 21, 2015·PLoS Neglected Tropical Diseases·Emilie LefoulonCoralie Martin

❮ Previous
Next ❯

Datasets Mentioned

BETA
MT135051-135093
MT151872-151910

Methods Mentioned

BETA
PCR

Software Mentioned

FigTree
DAMBE
Bioedit
ClustalX
MrBayes
MEGA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.