PMID: 7581519Nov 1, 1995Paper

Evaluation in tobacco of the organ specificity and strength of the rolD promoter, domain A of the 35S promoter and the 35S2 promoter

Transgenic Research
T Elmayan, M Tepfer

Abstract

In order to study the expression in plants of the rolD promoter of Agrobacterium rhizogenes, we have constructed chimaeric genes placing the coding region of the gusA (uidA) marker gene under control of two rolD promoter fragments of different length. Similar results were obtained with both genes. Expression studies were carried out in transformed R1 progeny plants. In mature transformed tobacco plants, the rolD-gus genes were expressed strongly in roots, and to much lower levels in stems and leaves. This pattern of expression was transmitted to progeny, though the ratio of the level of expression in roots relative to that in leaves was much lower in young seedlings. The degree of root specificity in rolD-gus transformants was less than that of a gene constructed with domain A of the CaMV 35S promoter, domA-gus, but the level of root expression was much higher than with the latter gene. However, the level of expression of the rolD-gus genes was less than that of a gus gene with a 35S promoter with doubled domain B, 35S2-gus. The rolD-gus genes had a distinctive pattern of expression in roots, compared to that of the two other genes, with the strongest GUS activity observed in the root elongation zone and in vascular tissue, and...Continue Reading

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Citations

Feb 8, 2011·Plant Cell Reports·Michel Edmond GhanemFrancisco Pérez-Alfocea
Sep 7, 2007·Molecular Genetics and Genomics : MGG·Katsutomo SasakiYuko Ohashi
Jun 7, 2008·Journal of Biosciences·Virupapuram VijaybhaskarImran Siddiqi
Oct 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Tomohiro ImamuraHideyuki Takahashi
Oct 27, 2010·Journal of Experimental Botany·Salah F Abou-ElwafaAndreas E Müller
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·Manjula GovindarajuluChristopher G Taylor
Aug 27, 2005·Genome Génome / Conseil National De Recherches Canada·Kerri B McIntosh, Peta C Bonham-Smith
Oct 17, 2008·Genome Génome / Conseil National De Recherches Canada·Je Hyeong JungYong Weon Seo
Jul 11, 2012·BMC Plant Biology·Junhua LiChangbin Chen
Apr 27, 2013·BMC Plant Biology·Reham M YoussefBenjamin F Matthews
Sep 7, 2011·BMC Research Notes·Niharika SharmaMohan B Singh
Sep 21, 2005·Genes & Genetic Systems·Takuro ItoAkira Kanno
Aug 27, 2013·PloS One·Hua BaiAndrea Polle
Mar 29, 2014·Plant Physiology and Biochemistry : PPB·Liang ZhangChongying Wang
Jun 12, 2012·Plant Science : an International Journal of Experimental Plant Biology·Fábio E SeverinoIvan G Maia
Nov 14, 2014·Applied Biochemistry and Biotechnology·Qing DongBeijiu Cheng
Sep 29, 2012·Plant Science : an International Journal of Experimental Plant Biology·Zhijian T LiDennis J Gray

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