Evaluation of 14-3-3 sigma as a potential partner of p16 in quiescence and differentiation

In Vitro Cellular & Developmental Biology. Animal
Payal AgarwalR Curtis Bird

Abstract

p16 is an important tumor suppressor gene encoded by the INK4A/ARF/INK4B gene locus that is conserved in humans, rodents, and canids. p16 regulates cell cycle in early G1 phase inhibiting transition out of cell cycle from G1/S phase by regulating a multi-protein control complex. p16-associated proteins, cyclin D, CDK4, and CDK6, experience expression level decreases or do not change during cell differentiation and quiescence in contrast to constant p16 expression in post-proliferative cell phases. We hypothesized that p16 has alternate binding partners, other than classical proliferation-associated proteins such as CDKs, in these post-proliferative cell phases. Using co-immunoprecipitation, we have identified 14-3-3σ as a potential alternate binding partner for p16 in quiescent post-proliferative canine mammary cancer cells. Additionally, expression of 14-3-3σ was maintained as fibroblasts exit cell cycle and differentiate to adipocytes simultaneously with continued expression of p16. Based on these results, we suggest that 14-3-3σ protein may be an alternative binding partner for p16 active during cell quiescence and may associate with p16 during cell differentiation.

References

Jan 1, 1992·Cancer Treatment and Research·D Tripathy, C C Benz
Nov 26, 2002·The Journal of Biological Chemistry·Helen RizosRichard F Kefford
Jan 23, 2004·Nature Reviews. Cancer·Heiko Hermeking
Oct 4, 2005·Nature Genetics·Bruce J HerronLinda D Siracusa
May 10, 2006·Biochemical and Biophysical Research Communications·Deepa BediRobert L Judd
May 16, 2006·Seminars in Cancer Biology·Mong-Hong Lee, Guillermina Lozano
May 16, 2006·Seminars in Cancer Biology·Heiko Hermeking, Anne Benzinger
Oct 24, 2007·Proteomics·Elielson Souza-RodríguesMontserrat Jaumot
Feb 26, 2009·Aging Cell·Ander MatheuManuel Serrano
Jun 5, 2010·The Journal of Biological Chemistry·Peng SunDhananjaya V Kalvakolanu

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Citations

Sep 21, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yun HuangWeiguo Huang

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Methods Mentioned

BETA
gene knockouts
biopsy
PCR
co-immunoprecipitation
electrophoresis
co-immunoprecipitations

Software Mentioned

NIH image scan

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