Evaluation of (188)Re-labeled NGR-VEGI protein for radioimaging and radiotherapy in mice bearing human fibrosarcoma HT-1080 xenografts

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Wenhui MaJing Wang

Abstract

Vascular endothelial growth inhibitor (VEGI) is an anti-angiogenic protein, which includes three isoforms: VEGI-174, VEGI-192, and VEGI-251. The NGR (asparagine-glycine-arginine)-containing peptides can specifically bind to CD13 (Aminopeptidase N) receptor which is overexpressed in angiogenic blood vessels and tumor cells. In this study, a novel NGR-VEGI fusion protein was prepared and labeled with (188)Re for radioimaging and radiotherapy in mice bearing human fibrosarcoma HT-1080 xenografts. Single photon emission computerized tomography (SPECT) imaging results revealed that (188)Re-NGR-VEGI exhibits good tumor-to-background contrast in CD13-positive HT-1080 tumor xenografts. The CD13 specificity of (188)Re-NGR-VEGI was further verified by significant reduction of tumor uptake in HT-1080 tumor xenografts with co-injection of the non-radiolabeled NGR-VEGI protein. The biodistribution results demonstrated good tumor-to-muscle ratio (4.98 ± 0.25) of (188)Re-NGR-VEGI at 24 h, which is consistent with the results from SPECT imaging. For radiotherapy, 18.5 MBq of (188)Re-NGR-VEGI showed excellent tumor inhibition effect in HT-1080 tumor xenografts with no observable toxicity, which was confirmed by the tumor size change and hematox...Continue Reading

References

Dec 1, 1990·Clinical Nuclear Medicine·J F EaryJ L Vanderheyden
Jan 1, 1996·Acta Oncologica·K J Kairemo
Mar 9, 2000·Nuclear Medicine and Biology·B T HsiehS J Wang
Mar 30, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Li-Jin ChewLu-Yuan Li
Nov 7, 2003·Journal of Clinical Immunology·Anjana Bhardwaj, Bharat B Aggarwal
Aug 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wen HouLu-Yuan Li
Aug 13, 2005·Nuclear Medicine Communications·Guillermina Ferro-FloresHéctor Garnica-Garza
Mar 7, 2006·Seminars in Ophthalmology·Linda J Metheny-Barlow, Lu-Yuan Li
May 30, 2006·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Guozheng LiuDonald J Hnatowich
Dec 11, 2007·Archives of Medical Research·Eugenio Torres-GarcíaPablo A Pichardo-Romero
Sep 18, 2009·Advances in Experimental Medicine and Biology·Gautam SethiBharat B Aggarwal
Apr 15, 2010·Current Topics in Medicinal Chemistry·Kai Chen, Xiaoyuan Chen
May 25, 2010·Nuclear Medicine Communications·Nalan Alan SelcukIlhami Uslu
Nov 3, 2010·Current Drug Discovery Technologies·David R JansenJan Rijn Zeevaart
Mar 6, 2012·Targeted Oncology·Lijiao DuanCunshuan Xu
Aug 16, 2012·Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists·Martin WernerMatthias Gutberlet
May 9, 2013·International Journal of Molecular Imaging·Maria ArgyrouMaria Lyra
Sep 18, 2013·Pharmaceutical Research·Fengxi LiuNa Zhang
Feb 1, 2014·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·B L FaintuchA M Moro
Feb 8, 2014·Theranostics·Yan XingKai Chen
Aug 8, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yahui ShaoJing Wang
Mar 4, 2015·Current Topics in Medicinal Chemistry·Rui HuangKai Chen

❮ Previous
Next ❯

Citations

Jul 27, 2018·Contrast Media & Molecular Imaging·Cynthia L Schreiber, Bradley D Smith
May 1, 2021·Pharmaceutics·Abiodun Ayo, Pirjo Laakkonen
Jun 1, 2021·PET Clinics·Sanaz KatalAli Gholamrezanezhad
Jun 3, 2021·International Journal of Molecular Sciences·Alexandru FloreaMatthias Bauwens

❮ Previous
Next ❯

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.