Abstract
A new fluorescent derivative of D-glucose, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), which had been previously developed for the analysis of glucose uptake activity by living cells, was investigated to evaluate its applicability for assaying the viability of yeast Candida albicans. Lineweaver-Burk plots showed to uptake of 2-NBDG to be competitively inhibited by D-glucose and not by L-glucose, which suggested the involvement of the glucose transporting system of C. albicans in the uptake of 2-NBDG. A good correlation was obtained between the yeast viability, determined by the plate-count method, and the 2-NBDG uptake activity of yeast cells (correlation constant: r = 0.97). This is expected to lead to the development of a new fluorescent probe for the determination of yeast cell viability.
References
Jan 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K H Jones, J A Senft
Oct 1, 1995·Applied and Environmental Microbiology·T Imai, T Ohno
Dec 1, 1993·Mycopathologia·A SanoM Miyaji
Feb 9, 1996·Biochimica Et Biophysica Acta·K YoshiokaH Matsuoka
Jan 1, 1994·Journal of Microbiological Methods·F P Yu, G A McFeters
Jul 1, 1992·Applied and Environmental Microbiology·K Autio, T Mattila-Sandholm
Sep 1, 1995·Applied and Environmental Microbiology·T TsujiS Tanaka
Jan 1, 1996·Applied and Environmental Microbiology·U KarstenG Holst
Citations
Aug 14, 2012·The Journal of Membrane Biology·Elham Raeisi, Lluis M Mir
Nov 2, 2007·Glycoconjugate Journal·M Carmen LouzaoLuis M Botana
Nov 15, 2005·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Roger G O'NeilNizar Mullani
Sep 3, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Mohamed HassaneinVito Quaranta
Sep 23, 2000·Journal of Microbiological Methods·F OrsiniS M Doglia
May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F LeiraM R Vieytes
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·K NielsenR S Boston
Jun 6, 2012·Sensors·Woong Hee KimDarren R Williams
Nov 10, 2013·PloS One·Narasimhan RajaramNirmala Ramanujam
Mar 31, 2015·PloS One·Adhiraj RoyJeong-Ho Kim
Nov 6, 2018·Natural Products and Bioprospecting·Yan-Ting LuWen-Yong Xiong
Sep 8, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J AchillesW Babel
Apr 21, 2016·Biochemistry·Junyi TaoTimothy J Hackmann
Apr 21, 2007·Bioconjugate Chemistry·Jelena LeviSanjiv Sam Gambhir