Evaluation of 99m Tc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors

Contrast Media & Molecular Imaging
Xiao ZhangXiaoli Lan

Abstract

Vascular cell adhesion molecule 1 (VCAM-1) is overexpressed in varieties of cancers. This study aimed to evaluate the application of a single chain variable fragment (scFv) of anti-VCAM-1 antibody labeled with 99mTc as a possible imaging agent in several tumors. VCAM-1 scFv was labeled with 99mTc using succinimidyl 6-hydrazinium nicotinate hydrochloride, and 99mTc-HYNIC-VCAM-1scFv was successfully synthesized with a high radiolabeling yield. VCAM-1 expression was evaluated in six cell lines by immunofluorescence staining. In vitro binding assays showed different binding affinities of 99mTc-HYNIC-VCAM-1scFv in different tumor cell lines, with high uptake in B16F10 melanoma and HT1080 fibrosarcoma cells, which was consistent with immunofluorescence staining results. In vivo SPECT planar imaging demonstrated that B16F10 and HT1080 tumors could be clearly visualized. Less intense uptake was observed in human SKOV3.ip ovarian tumor, and weak uptake was observed in human A375m melanoma, MDA-MB-231 breast cancer, and 786-O renal tumors. These findings were confirmed by biodistribution and immunofluorescence studies. High uptake by B16F10 tumors was inhibited by excess unlabeled VCAM-1scFv. 99mTc-HYNIC-VCAM-1scFv, which selectively bin...Continue Reading

References

Dec 21, 2006·Toxicologic Pathology·Xiaoyou Ying, Thomas M Monticello
Oct 17, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Beat A KaufmannJonathan R Lindner
Feb 6, 2010·Expert Review of Anticancer Therapy·Eleni Andreopoulou, Massimo Cristofanilli
Feb 26, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·João D G CorreiaIsabel Santos
Feb 26, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Levente K MeszarosPhilip J Blower
Aug 11, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qing Chen, Joan Massagué
Sep 14, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Jennifer M ScaliciJill K Slack-Davis
Dec 7, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Faruk TasDerya Duranyildiz
Jan 16, 2014·The Journal of Investigative Dermatology·Christoph M Hammers, John R Stanley
Apr 29, 2014·International Journal of Cancer. Journal International Du Cancer·Martin Schlesinger, Gerd Bendas
Jul 24, 2014·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Laura E Ott, Susan Carson
Aug 27, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Alexis BroisatCatherine Ghezzi
Apr 29, 2016·Journal of Labelled Compounds & Radiopharmaceuticals·Chongjiao LiXiaoli Lan

❮ Previous
Next ❯

Citations

Apr 22, 2020·Journal of Drug Targeting·Dongjian ZhangJian Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
phage display
xenograft
xenografts
ELISA

Software Mentioned

Statistical Package for the Social Sciences ( SPSS )
SPSS
ImageJ
GraphPad Prism

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

The Quarterly Journal of Nuclear Medicine and Molecular Imaging : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR), [and] Section of the Society of Radiopharmaceutica
Yu LiuRui An
© 2022 Meta ULC. All rights reserved