Evaluation of a CD61 MoAb method for enumeration of platelets in thrombocytopenic patients and its impact on the transfusion decision-making process

Transfusion
J L ArroyoA Orfao

Abstract

Almost all automated hematology cell analyzers use methods based on either the impedance (PLTi) or the optical (PLTo) properties of the cells for performing platelet counts. To improve the accuracy of platelet counts in peripheral blood (PB), the use of CD61 (GPIIIa) MoAbs (ImmunoPLT method) has recently been introduced in an automated hematology blood-analyzer system (Cell-Dyn 4000, Abbott Diagnostics). A comparative evaluation was made of the accuracy and precision of the three methods currently available in the Cell-Dyn 4000 automated hematology cell analyzer for counting the number of platelets per microliter of PB in a total of 47 patients with chemotherapy-induced thrombocytopenia. A flow cytometric PB platelet count was also performed in parallel and used as an external reference. PB platelet counts showed a good correlation among the PLTo, CD61-ImmunoPLT, and flow cytometric methods. In contrast, the PLTi procedure usually provided an overestimation of the number of platelets per microliter. Although a good correlation was observed between the flow cytometric reference method and both the ImmunoPLT and PLTo methods, the highest degree of agreement was found for the ImmunoPLT techniques (94% vs. 67%). A comparative analy...Continue Reading

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Citations

Mar 4, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Robert MüllerColin Stephen Scott
Jul 29, 2005·Clinical and Laboratory Haematology·P FelleF Ni Chonchubhair
May 27, 2005·Cytometry. Part B, Clinical Cytometry·Paul HarrisonSam Machin
Aug 30, 2007·Transfusion·Margriet J Dijkstra-TiekstraJanny de Wildt-Eggen
Dec 18, 2013·Vox Sanguinis·M LozanoUNKNOWN Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Sep 21, 2013·American Journal of Clinical Pathology·Margreet SchoorlJohannes van Pelt
Jul 16, 2019·International Journal of Laboratory Hematology·Yolien Van der BekenKristin Jochmans

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