PMID: 11318370Apr 25, 2001Paper

Evaluation of a commercial ELISA for diagnosis of paratuberculosis in cattle

Journal of the American Veterinary Medical Association
D A DargatzJudith R Stabel

Abstract

To evaluate sensitivity and specificity of a new ELISA for antibodies against Mycobacterium avium subsp paratuberculosis. Cross-sectional observational survey. Serum samples from 590 cattle that were infected with M avium subsp paratuberculosis and 723 cattle that were not infected. Serum samples were tested by use of an ELISA for antibodies against M avium subsp paratuberculosis. Sensitivity of the test varied from 15.4 to 88.1%, depending on the clinical stage and bacterial shedding status of the cattle. Results obtained with use of the new ELISA agreed favorably with those of a previous ELISA. Practitioners must be aware of variability in the sensitivity of the test, which depends on the clinical and shedding status of the cattle, because this may affect interpretation of test results.

References

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Citations

May 25, 2004·Preventive Veterinary Medicine·Maarten F WeberMirjam Nielen
Aug 28, 2003·Preventive Veterinary Medicine·G van SchaikJ A VanLeeuwen
Jul 29, 2011·Clinical and Vaccine Immunology : CVI·Dairu ShuBryce M Buddle
May 6, 2005·Journal of Clinical Microbiology·Alifiya S MotiwalaSrinand Sreevatsan
Dec 20, 2012·Veterinary Medicine International·Patrick Pithua, Nathaniel S Kollias
Dec 17, 2009·Preventive Veterinary Medicine·Richard BennettIan McFarlane
Jun 18, 2002·The Veterinary Clinics of North America. Food Animal Practice·David R Smith
Apr 7, 2010·Preventive Veterinary Medicine·J B OsterstockN D Cohen
Nov 30, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S L B McKennaH W Barkema
Mar 29, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Raymond W SweeneyTerry Fyock
May 11, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Solis NortonCord Heuer
Mar 13, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Patrick PithuaJudith R Stabel
Feb 21, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Tim E CarpenterRobert H Whitlock
May 8, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M Paulina FryMichael T Collins
Oct 16, 2004·Journal of Dairy Science·S L B McKennaD C Sockett
Aug 24, 2001·Journal of the American Veterinary Medical Association·D A DargatzS J Wells
Aug 25, 2006·Clinical and Vaccine Immunology : CVI·Scott J WellsRobert H Whitlock
Jun 9, 2005·Clinical and Diagnostic Laboratory Immunology·Michael T CollinsRobert H Whitlock

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