Evaluation of a commercial kit for measuring vitellogenin in the fathead minnow (Pimephales promelas)

Ecotoxicology and Environmental Safety
Kathleen M Jensen, Gerald T Ankley

Abstract

Vitellogenin (vtg) concentrations in oviparous animals such as fish represent an integrated indicator of the status of the reproductive endocrine system. As such, vtg is a common measurement endpoint in tests designed to detect certain classes of endocrine-disrupting chemicals (EDCs). The most common approach to measuring vtg is via enzyme-linked immunosorbent assays (ELISAs). However, because labs testing EDCs in fish often use slightly different ELISAs (e.g., in terms of antibodies, binding antigens, standards), results among studies are not always comparable. One approach to obviating this would be for researchers to use standardized ELISA kits from a common source(s). The fathead minnow (Pimephales promelas) is a small fish model commonly used for EDC testing. The purpose of this study was to evaluate a recently developed commercial ELISA kit for measuring vtg in the fathead minnow. The commercial ELISA, based on a monoclonal antibody to fathead minnow vtg, was compared to an ELISA that utilizes a fathead minnow polyclonal antibody, which has been used extensively in our lab and others for several years. Plasma samples for this comparison came from three studies in which fathead minnows had been exposed to different model E...Continue Reading

References

Oct 1, 1995·Environmental Health Perspectives·J P Sumpter, S Jobling
Aug 26, 1998·Critical Reviews in Toxicology·C R TylerJ P Sumpter
Aug 12, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·L G ParksC V Sullivan
Apr 19, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gerald T AnkleyRichard L Leino
Nov 13, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Charles R TylerEduarda M Santos
May 20, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gerald T AnkleyVickie S Wilson
Feb 1, 2006·Environmental Toxicology and Chemistry·Peter A Van VeldLance T Yonkos
Jul 5, 2006·Environmental Health Perspectives·Thomas H HutchinsonCharles R Tyler

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Citations

Jul 31, 2012·The Annals of Occupational Hygiene·Gunnar D NielsenLars K Poulsen
Nov 13, 2013·Fish Physiology and Biochemistry·Xiaozheng ZhangSiyu Mao
Jan 3, 2014·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Katherine Kemler CoadyTroy Alan Marino
Apr 18, 2009·Toxicology and Applied Pharmacology·Naida YinZhan Yin
Jul 16, 2008·Molecular and Cellular Endocrinology·Stefan Scholz, Ian Mayer
Nov 26, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Henrik HolbechAnne Gourmelon
Nov 28, 2013·Critical Reviews in Toxicology·Janice E ChambersGlen Van Der Kraak
May 10, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Andrew M CowieChristopher J Martyniuk

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