Evaluation of a digital microfluidic real-time PCR platform to detect DNA of Candida albicans in blood.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
W A SchellT G Mitchell

Abstract

Species of Candida frequently cause life-threatening infections in neonates, transplant and intensive care unit (ICU) patients, and others with compromised host defenses. The successful management of systemic candidiasis depends upon early, rapid diagnosis. Blood cultures are the standard diagnostic method, but identification requires days and less than half of the patients are positive. These limitations may be eliminated by using real-time polymerase chain reaction (PCR) to detect Candida DNA in the blood specimens of patients at risk. Here, we optimized a PCR protocol to detect 5-10 yeasts in low volumes of simulated and clinical specimens. We also used a mouse model of systemic candidiasis and determined that candidemia is optimally detectable during the first few days after infection. However, PCR tests are often costly, labor-intensive, and inconvenient for routine use. To address these obstacles, we evaluated the innovative microfluidic real-time PCR platform (Advanced Liquid Logic, Inc.), which has the potential for full automation and rapid turnaround. Eleven and nine of 16 specimens from individual patients with culture-proven candidemia tested positive for C. albicans DNA by conventional and microfluidic real-time PC...Continue Reading

References

Nov 1, 1991·Mayo Clinic Proceedings·A TelentiG D Roberts
Jan 1, 1996·Journal of Clinical Microbiology·A J van DeventerH A Verbrugh
Aug 1, 1996·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Hanneke J. van DeventerHenry A. Verbrugh
Apr 18, 2003·The New England Journal of Medicine·Greg S MartinMarc Moss
Jul 5, 2003·Journal of Clinical Microbiology·Younes MaaroufiFrançoise Crokaert
Mar 17, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P M OlaecheaUNKNOWN EPCAN Study Group
Apr 22, 2004·Lab on a Chip·M G PollackR B Fair
Jan 7, 2006·Pediatrics·Daniel K BenjaminUNKNOWN National Institute of Child Health and Human Development Neonatal Research Network
May 2, 2006·International Journal of Antimicrobial Agents·Anna Maria TortoranoRenee Grillot
Jan 16, 2007·Clinical Microbiology Reviews·M A Pfaller, D J Diekema
Sep 22, 2007·Medical Mycology·A CarvalhoF Rodrigues
Feb 12, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R McMullanR J Hay
Nov 22, 2008·Lab on a Chip·Ramakrishna SistaVamsee Pamula
Feb 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Leah E CowenSusan Lindquist
May 7, 2009·FEMS Microbiology Letters·P Lewis WhiteRosemary A Barnes
Jun 17, 2009·Journal of Medical Microbiology·Nele WellinghausenSusanne Gebert
Feb 16, 2010·Analytical Chemistry·Zhishan HuaMichael G Pollack
Mar 17, 2010·Diagnostic Microbiology and Infectious Disease·Elizabeth Wulff-BurchfieldThomas G Mitchell
May 11, 2010·Current Fungal Infection Reports·L Corbin DowneyMichael Cohen-Wolkowiez
Aug 20, 2010·Journal of Clinical Microbiology·P Lewis WhiteUNKNOWN European Aspergillus PCR Initiative
Nov 26, 2010·Journal of Clinical Microbiology·Tomer AvniMical Paul
May 7, 2011·Expert Review of Molecular Diagnostics·Michael G PollackAllen E Eckhardt
Jun 17, 2011·Journal of Clinical Microbiology·Christopher D PfeifferBarbara D Alexander
Jul 14, 2011·Critical Reviews in Microbiology·Joe L HsuDavid A Stevens
Sep 13, 2011·Annals of Intensive Care·Philippe EggimannOscar Marchetti

❮ Previous
Next ❯

Citations

Nov 24, 1999·Biochimica Et Biophysica Acta·G L WallisJ F Peberdy
Apr 18, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·A SchultzJ Heikenfeld
Nov 13, 2012·Journal of Microbiological Methods·Timothy StraubCynthia J Bruckner-Lea
Sep 22, 2015·International Journal of Molecular Sciences·Jie-Long HeShih-Kang Fan
May 3, 2013·Current Medical Research and Opinion·John R Perfect
Dec 19, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Peter G PappasJack D Sobel
Nov 25, 2014·Journal of Medical Microbiology·Fernando Teles, Jorge Seixas
Jan 15, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cornelius J Clancy, M Hong Nguyen
Apr 20, 2016·Critical Reviews in Biotechnology·Mohammadali SafaviehWaseem Asghar
Sep 30, 2016·Frontiers in Microbiology·Zheng-Xin HeFu-Kun Wang
Jul 2, 2014·Clinical Microbiology Reviews·Marios ArvanitisEleftherios Mylonakis
Sep 4, 2014·Environmental Monitoring and Assessment·Pauline DusseuxLaurence Hubert-Moy
May 30, 2013·Journal of Neural Engineering·Jordan J WilliamsDaniel W Moran
Jul 5, 2013·Lab on a Chip·Maïwenn Kersaudy-Kerhoas, Elodie Sollier
Aug 23, 2017·Expert Review of Anti-infective Therapy·Wilfried PoschCornelia Lass-Flörl
May 23, 2014·Expert Review of Anti-infective Therapy·Cornelius J Clancy, Minh Hong Nguyen
Jul 5, 2017·Sensors·Beatriz CoelhoPedro V Baptista
Feb 13, 2020·Revista Do Instituto De Medicina Tropical De São Paulo·Felipe Delatorre BusserVera Lucia Teixeira de Freitas
Nov 30, 2018·Biosensors & Bioelectronics·Fenxiang ZouChaoyong James Yang
Jan 15, 2021·Journal of Fungi·Lewis P White, Jessica S Price
Jun 29, 2021·Current Fungal Infection Reports·P Lewis WhiteMatthijs Backx
May 14, 2019·ACS Omega·Waseem AsgharUtkan Demirci
Oct 5, 2021·Frontiers in Microbiology·Biao ChenDongmei Shi

❮ Previous
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