Evaluation of a direct immunofluorescence cytospin assay for the detection of herpes simplex virus in clinical samples

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
J ReinaI Blanco

Abstract

A comparison between a direct immunofluorescence assay (DFA) and the shell-vial culture (SVC) was conducted to evaluate their efficacies according to the quality and origin of the sample and the type of herpes simplex (HSV) responsible for the infection. The SVC detected all 58 HSV-infected samples, while the DFA detected only 49 (84.5%) positive samples. The DFA detected HSV type 1 in 22 of 89 samples (24.7%) and HSV type 2 in 27 of 96 samples (28.1%). Compared with the SVC, the DFA had a sensitivity of 75.8% for HSV type 1 and 93.1% for HSV type 2. The sensitivity of the DFA depends on the quality of the sample. Thus, while DFA is recommendable as a screening method, the SVC remains the method of choice for obtaining the maximum diagnostic yield from the sample.

References

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Citations

Apr 10, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·David M WhileyTheo P Sloots
Jan 8, 2000·Diagnostic Microbiology and Infectious Disease·L M ScicchitanoP P Bourbeau
Oct 18, 2013·Journal of Clinical Microbiology·Neil W AndersonNathan A Ledeboer
Oct 9, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J ReinaM Munar
Jul 14, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·A Chantal CavinessGail J Demmler-Harrison
Feb 14, 2017·Pediatric Annals·Amy WangJulia Rosebush
Aug 16, 2019·Journal of Clinical Microbiology·Yi-Wei Tang
Jun 24, 2016·Microbiology Spectrum·Myron J LevinD Scott Schmid

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