Evaluation of a four-channel multiplexed electrospray triple quadrupole mass spectrometer for the simultaneous validation of LC/MS/MS methods in four different preclinical matrixes

Analytical Chemistry
L YangP J Rudewicz

Abstract

A four-channel multiplexed electrospray interface on a triple quadrupole mass spectrometer was evaluated for the simultaneous validation of LC/MS/MS methods for the quantitation of loratadine and its metabolite, descarboethoxyloratadine, in four different biological matrixes. The assays were performed in rat, rabbit, mouse, and dog plasma from 1 to 1000 ng/mL using 96-well solid-phase extraction for sample preparation. The limit of quantitation of 1 ng/mL corresponded to 5.56 pg of each analyte injected on-column. For the drug, quality control samples (n = 6 at four concentrations) had precision ranging from 0.967 to 16.0% and accuracy ranging from -8.44 to 10.5% across all four species. For the metabolite, the precision ranged from 0.684 to 11.0% and the accuracy was between 6.36 and -9.06%. Intersprayer cross talk for the multiplexed electrospray ion source was evaluated as a function of analyte concentration and was less than 0.08% at concentrations as high as 1000 ng/mL. These results demonstrate the feasibility of using parallel analysis to reduce the time required for method validation and to increase sample throughput in drug development studies.

References

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Citations

Jan 24, 2004·Journal of Pharmaceutical and Biomedical Analysis·Isam Ismail SalemJaafar I Al Tamimi
Sep 27, 2005·Journal of the American Society for Mass Spectrometry·Yu XiaScott A McLuckey
Jun 7, 2002·Journal of Pharmaceutical and Biomedical Analysis·Weng NaidongTimothy D J Halls
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Feb 19, 2010·Expert Opinion on Drug Metabolism & Toxicology·Wilson Z Shou, Jun Zhang
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Apr 3, 2016·Biomedical Chromatography : BMC·Maria KatselouSotiris Athanaselis
Aug 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jiwen ChenPatrick J Rudewicz
Sep 11, 2002·Rapid Communications in Mass Spectrometry : RCM·Russell P GrantShelley Mackenzie-McMurter
Aug 28, 2001·Journal of Mass Spectrometry : JMS
Oct 4, 2002·Rapid Communications in Mass Spectrometry : RCM·Bradley B SchneiderDavid D Y Chen
May 26, 2005·Rapid Communications in Mass Spectrometry : RCM·Laurent LeclercqJosé Castro-Perez
Dec 17, 2005·Electrophoresis·Eric BonneilPierre Thibault
Nov 27, 2004·Rapid Communications in Mass Spectrometry : RCM·Linda E ChovanYau Yi Lau
Mar 27, 2019·The Analyst·Hiroka KishiHajime Ohtani
Feb 1, 2021·Analytica Chimica Acta·Thomas ThemelisSebastiaan Eeltink
Apr 30, 2003·Journal of Mass Spectrometry : JMS·R KostiainenS Auriola

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