Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples

Clinical and Diagnostic Laboratory Immunology
Yousif Al-YousifSanjay Kapil

Abstract

The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect the presence of group A rotavirus antigen. Of the 63 samples screened by VI-FA, 33 (58%) tested positive for rotavirus antigen. When the results from the LAT were compared to those from VI-FA, the "gold standard" for detection of bovine rotavirus in fecal samples, the sensitivity and specificity were found to be 87.8 and 73.3%, respectively. Latex agglutination compared with ELISA (the reference method) showed 100% sensitivity and 96.3% specificity, and when ELISA was compared with VI, the sensitivity was 84.8% and the specificity was 73.3%. Latex agglutination is easy to perform in a short time and does not require expensive equipment or skilled personnel, and the reagents have long shelf lives. These factors make the LAT suitable and highly efficient for use in a clinical laboratory as a rapid screening test for bovine rotavirus.

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Citations

Aug 28, 2010·The Journal of Microbiology·Heetae LeeGwangPyo Ko
Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·Yashpal Singh MalikManmohan Parida
Feb 18, 2004·Journal of Microbiological Methods·Shaw-Jye WuClarence I Kado
Jan 9, 2003·Journal of Clinical Microbiology·Roger K MaesRobert Holland
Jul 2, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L RodákL Valícek
Jan 10, 2013·Microbial Biotechnology·Raweewan ThiramanasDuangporn Polpanich
Jan 1, 2012·Saudi Journal of Biological Sciences·Meshref A Al-RuwailiSamy A Selim
Apr 30, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·Pablo Weilg EspejoJuana Del Valle Mendoza

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