Evaluation of a modified APTT-based method for determination of APC resistance in plasma from patients on heparin or oral anticoagulant therapy

Thrombosis Research
C HallS Rosén

Abstract

An APTT-based kit method (Coatest APC Resistance), modified by predilution 1+4 of sample plasma in a plasma diluent containing a heparin antagonist (V-DEF plasma), has been evaluated on plasmas from patients treated with unfractionated (n = 110) or either of three different low molecular heparins (n=44), or with oral anticoagulants (n=147). Irrespective of treatment, no difference was observed in the APC response as compared to untreated individuals (n=62), and a complete discrimination was obtained between individuals with a normal factor V genotype and those carrying the FV:Q506 mutation. Furthermore, in contrast to the original, APTT-based kit method, where anticoagulant therapy results in a prolongation of the APTT, the modified kit provided APTT values within the normal range for orally anticoagulated (INR< or =6) and for all heparin treated (< or =1 IU/mL) patients except for one with a suspected presence of phospholipid antibodies. Due to the predilution in V-DEF plasma, contamination with platelets up to 1.5 x 10(4)/microL had a negligible effect on analysis of frozen plasmas regarding their classification as normal or abnormal. Analyses of fresh plasmas show no influence at platelet counts up to 6x10(4)/microL. Consequ...Continue Reading

References

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Citations

Mar 12, 2003·Hematology/oncology Clinics of North America·Gerry A F Nicolaes, Björn Dahlbäck
Nov 9, 2010·American Journal of Obstetrics and Gynecology·Ulla KjellbergMargareta Hellgren
Sep 19, 2017·Archives of Pathology & Laboratory Medicine·Elena MaryamchikElizabeth M Van Cott
Apr 11, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·M van der Neut KolfschotenR M Bertina
Sep 30, 2006·World Journal of Gastroenterology : WJG·Gottfried NovacekHarald Vogelsang
Feb 27, 2020·Archives of Pathology & Laboratory Medicine·Elena Maryamchik, Elizabeth M Van Cott

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