Abstract
The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.
References
Feb 1, 1990·Journal of Clinical Microbiology·S M CallisterJ L Ellingson
Aug 1, 1989·Journal of Clinical Microbiology·R J Sinsky, J Piesman
Aug 15, 1970·Nature·U K Laemmli
Oct 1, 1994·International Journal of Systematic Bacteriology·D PosticG Baranton
Feb 1, 1996·Medical Microbiology and Immunology·B WilskeV Preac-Mursic
Sep 21, 2000·Memórias do Instituto Oswaldo Cruz·I S AbelT T Schumaker
Nov 4, 2000·Journal of Clinical Microbiology·A R MarquesV Gill
Oct 16, 2001·Journal of Medical Microbiology·E Ruzić-SabljićF Strle
Mar 17, 2004·Vector Borne and Zoonotic Diseases·Fatima JoudaLise Gern
Oct 27, 2004·Emerging Infectious Diseases·Islay RodríguezOmar Fuentes
Dec 15, 2004·International Journal of Medical Microbiology : IJMM·Eva Ruzić-Sabljić, Franc Strle
Jan 5, 2006·Applied and Environmental Microbiology·Poupon Marie-AngèleLise Gern
Jan 13, 2006·Folia Parasitologica·Libor GrubhofferJames H Oliver
Mar 15, 2006·International Journal of Medical Microbiology : IJMM·Eva Ruzić-SabljićFranc Strle
Dec 13, 2006·Wiener klinische Wochenschrift·Allen C Steere
Citations
Mar 9, 2013·International Journal of Medical Sciences·Eva SapiBruce A Kuo
Jun 1, 2016·Frontiers in Microbiology·Jie FengYing Zhang
Sep 14, 2010·Memórias do Instituto Oswaldo Cruz·Angela de OliveiraNatalino Hajime Yoshinari